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Literature summary for 6.3.4.5 extracted from

  • Shen, L.J.; Lin, W.C.; Beloussow, K.; Shen, W.C.
    Resistance to the anti-proliferative activity of recombinant arginine deiminase in cell culture correlates with the endogenous enzyme, argininosuccinate synthetase (2003), Cancer Lett., 191, 165-170.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the resistance to this treatment with the AS activity in the cell lines Homo sapiens
recombinant mycoplasma arginine deiminase 350% increase in AS activity in HeLa cells after rADI treatment, no activity change in umbilical vein endothelial cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, correlation of the low resistance to this treatment with a low AS activity in the sensitive cell line Canis lupus familiaris
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the high resistance to this treatment with a high AS activity in the cell line Mus musculus
recombinant mycoplasma arginine deiminase 36% decrease in AS activity after rADI treatment Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-citrulline + L-aspartate Mus musculus the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate Homo sapiens the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate Canis lupus familiaris the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000001
-
37°C, cell homogenate Canis lupus familiaris
0.000006
-
37°C, A-549 cell homogenate Homo sapiens
0.000013
-
37°C, LNCaP cell homogenate Homo sapiens
0.000019
-
37°C, MCF-7 cell homogenate Homo sapiens
0.000048
-
37°C, HeLa cell homogenate Homo sapiens
0.000174
-
37°C, cell homogenate Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid Mus musculus AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid Homo sapiens AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine Mus musculus AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine Homo sapiens AMP + diphosphate + L-argininosuccinate
-
?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
?

Synonyms

Synonyms Comment Organism
Argininosuccinate synthetase
-
Mus musculus
Argininosuccinate synthetase
-
Homo sapiens
Argininosuccinate synthetase
-
Canis lupus familiaris
AS
-
Mus musculus
AS
-
Homo sapiens
AS
-
Canis lupus familiaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Homo sapiens
37
-
assay at Canis lupus familiaris