Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6 bisphosphate | binds to the Asp loop and induces a conformation that facilitates aspartate binding to the enzyme active site | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
K62L | increase in Km values for IMP, GTP and aspartate, respectively | Plasmodium falciparum |
N429V | increase in Km values for IMP, GTP and aspartate, respectively, along with a 5 fold drop in the kcat value | Plasmodium falciparum |
R155A | residue influences both ligand binding and catalysis | Plasmodium falciparum |
R155A/G146R | unlike dimeric wild-type, mainly monomeric. Inactive | Plasmodium falciparum |
R155K | residue influences both ligand binding and catalysis | Plasmodium falciparum |
R155L | residue influences both ligand binding and catalysis | Plasmodium falciparum |
T307V | increase in Km values for IMP, GTP and aspartate, respectively | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | competitive with aspartate | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0167 | - |
IMP | wild-type, pH 7.0, 37°C | Plasmodium falciparum | |
0.0205 | - |
IMP | wild-type, pH 7.0, 25°C | Plasmodium falciparum | |
0.0216 | - |
IMP | mutant K62L, pH 7.0, 37°C | Plasmodium falciparum | |
0.0248 | - |
GTP | wild-type, pH 7.0, 37°C | Plasmodium falciparum | |
0.027 | - |
GTP | wild-type, pH 7.0, 25°C | Plasmodium falciparum | |
0.027 | - |
GTP | mutant N429V, pH 7.0, 37°C | Plasmodium falciparum | |
0.0278 | - |
GTP | mutant R155L, pH 7.0, 25°C | Plasmodium falciparum | |
0.0285 | - |
IMP | mutant T307V, pH 7.0, 37°C | Plasmodium falciparum | |
0.0347 | - |
GTP | mutant T307V, pH 7.0, 37°C | Plasmodium falciparum | |
0.0386 | - |
GTP | mutant R155K, pH 7.0, 25°C | Plasmodium falciparum | |
0.0465 | - |
GTP | mutant K62L, pH 7.0, 37°C | Plasmodium falciparum | |
0.0497 | - |
IMP | mutant N429V, pH 7.0, 37°C | Plasmodium falciparum | |
0.31 | - |
L-Asp | wild-type, pH 7.0, 37°C | Plasmodium falciparum | |
0.37 | - |
L-Asp | wild-type, pH 7.0, 25°C | Plasmodium falciparum | |
0.48 | - |
L-Asp | mutant R155K, pH 7.0, 25°C | Plasmodium falciparum | |
0.557 | - |
IMP | mutant R155K, pH 7.0, 25°C | Plasmodium falciparum | |
0.57 | - |
L-Asp | mutant N429V, pH 7.0, 37°C | Plasmodium falciparum | |
0.78 | - |
L-Asp | mutant K62L, pH 7.0, 37°C | Plasmodium falciparum | |
1.1 | - |
L-Asp | mutant T307V, pH 7.0, 37°C | Plasmodium falciparum | |
1.32 | - |
L-Asp | mutant R155L, pH 7.0, 25°C | Plasmodium falciparum | |
2.554 | - |
IMP | mutant R155L, pH 7.0, 25°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration, mutant R155A/G146R, plus small peak of dimeric enzyme | Plasmodium falciparum |
50000 | - |
2 * 50000, calculated, wild-type | Plasmodium falciparum |
92000 | - |
gel filtration, wild-type and mutants N429V, K62L, T307V, R155V, R155K, R155A | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q9U8D3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-Asp | - |
Plasmodium falciparum | GDP + phosphate + adenylosuccinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, calculated, wild-type | Plasmodium falciparum |
More | conserved arginine residue R155 is involved in dimer crosstalk and interacts with IMP in the active site of the symmetry related subunit | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
IMP | mutant R155A, pH 7.0, 25°C | Plasmodium falciparum | |
0.01 | - |
IMP | mutant R155L, pH 7.0, 25°C | Plasmodium falciparum | |
0.112 | - |
IMP | mutant R155K, pH 7.0, 25°C | Plasmodium falciparum | |
0.32 | - |
IMP | mutant N429V, pH 7.0, 37°C | Plasmodium falciparum | |
1 | - |
IMP | wild-type, pH 7.0, 25°C | Plasmodium falciparum | |
1.09 | - |
IMP | mutant K62L, pH 7.0, 37°C | Plasmodium falciparum | |
1.44 | - |
IMP | wild-type, pH 7.0, 37°C | Plasmodium falciparum | |
1.72 | - |
IMP | mutant T307V, pH 7.0, 37°C | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
phosphate | pH 7.0, 37°C | Plasmodium falciparum |