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Literature summary for 6.3.4.4 extracted from
- Methanocaldococcus jannaschii adenylosuccinate synthetase: Studies on temperature dependence of catalytic activity and structural stability (2008), Biochim. Biophys. Acta, 1784, 2019-2028.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 (DE3) cells | Methanocaldococcus jannaschii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Methanocaldococcus jannaschii |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
4 * 38000, gel filtration and dynamic light scattering, AdSS exhibits dimer-tetramer equilibrium with the equilibrium shifting towards the dimer in the presence of 100 mM NaCl | Methanocaldococcus jannaschii |
| 79000 | - |
2 * 79000, gel filtration, AdSS exhibits dimer-tetramer equilibrium with the equilibrium shifting towards the dimer in the presence of 100 mM NaCl | Methanocaldococcus jannaschii |
| 109000 | - |
gel filtration, in the absence of denaturant AdSS elutes as a single peak corresponding to a molecular mass of 109000 Da, which is indicative of dimer-tetramer equilibrium | Methanocaldococcus jannaschii |
| 160000 | - |
gel filtration, in 20 mM Tris-HCl, pH 7.4 | Methanocaldococcus jannaschii |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | dramatic loss in secondary structure above 4 M guanidine-HCl with complete loss in secondary structure at 5 M guanidine-HCl | Methanocaldococcus jannaschii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanocaldococcus jannaschii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + IMP + L-Asp | - |
Methanocaldococcus jannaschii | GDP + phosphate + adenylosuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 79000, gel filtration, AdSS exhibits dimer-tetramer equilibrium with the equilibrium shifting towards the dimer in the presence of 100 mM NaCl | Methanocaldococcus jannaschii |
| tetramer | 4 * 38000, gel filtration and dynamic light scattering, AdSS exhibits dimer-tetramer equilibrium with the equilibrium shifting towards the dimer in the presence of 100 mM NaCl | Methanocaldococcus jannaschii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Adenylosuccinate synthetase | - |
Methanocaldococcus jannaschii |
| AdSS | - |
Methanocaldococcus jannaschii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 90 | thermal unfolding of AdSS exhibits a melting temperature of 85°C with the process being only partially reversible, the half life of inactivation is 340 min, 150 min, 110 min, 60 min, 30 min and 15 min at 65, 70, 75, 80, 85 and 90°C, respectively | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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