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Literature summary for 6.3.4.4 extracted from
- Purification and characterization of recombinant Plasmodium falciparum adenylosuccinate synthetase expressed in Escherichia coli (2002), Protein Expr. Purif., 25, 65-72.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | most parasitic protozoa lack the de novo purine biosynthetic pathway and rely exclusively on the salvage pathway for their purine biosynthetic pathway, enzymes of the salvage pathway are candidate drug targets | Plasmodium falciparum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| hyperexpressed in Escherichia coli BL21 (DE3), complementation of purA mutant strain H1238 | Plasmodium falciparum |
General Stability
| General Stability | Organism |
|---|---|
| requires presence of dithiothreitol during the entire course of purification for activity | Plasmodium falciparum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
GTP | pH 7.5, 37°C | Plasmodium falciparum |  |
| 0.0228 | - |
IMP | pH 7.5, 37°C | Plasmodium falciparum | |
| 1.4 | - |
aspartate | pH 7.5, 37°C | Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, recombinant protein, SDS-PAGE | Plasmodium falciparum |
| 105000 | - |
gel filtration | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + IMP + L-aspartate | Plasmodium falciparum | salvage pathway | GDP + phosphate + adenylosuccinate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1144 | - |
- |
Plasmodium falciparum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, glycerol and EDTA stabilizes enzyme activity during storage, stable for at least 3 weeks | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + IMP + L-aspartate | - |
Plasmodium falciparum | GDP + phosphate + adenylosuccinate | - |
? | |
| GTP + IMP + L-aspartate | salvage pathway | Plasmodium falciparum | GDP + phosphate + adenylosuccinate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 50000, recombinant protein, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Adenylosuccinate synthetase | - |
Plasmodium falciparum |
| AdSS | - |
Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Plasmodium falciparum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 8.8 | - |
Plasmodium falciparum |
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