Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.4.4 extracted from

  • Honzatko, R.B.; Stayton, M.M.; Fromm, H.J.
    Adenylosuccinate synthetase: Recent developments (1999), Adv. Enzymol. Relat. Areas Mol. Biol., 73, 57-102.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cDNA Dictyostelium discoideum
gene ade2 Schizosaccharomyces pombe
mouse muscle gene AdSS1 and mouse nonmuscle gene AdSS2 Mus musculus
purA gene Acidithiobacillus ferrooxidans
purA gene Haemophilus influenzae
purA gene Bacillus subtilis
purA gene overexpressed in strain H1238 Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

Protein Variants

Protein Variants Comment Organism
R143K site-directed mutagenesis Escherichia coli
R143L site-directed mutagenesis Escherichia coli
R303L site-directed mutagenesis Escherichia coli
R304L site-directed mutagenesis Escherichia coli
R305L site-directed mutagenesis Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Hadacidin
-
Escherichia coli
Hadacidin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
GTP
-
Escherichia coli
0.01
-
L-aspartate
-
Escherichia coli
0.02
-
IMP
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ absolute requirement for divalent metal ions Escherichia coli
Ca2+ absolute requirement for divalent metal ions Escherichia coli
Co2+ absolute requirement for divalent metal ions Escherichia coli
Cu2+ absolute requirement for divalent metal ions Escherichia coli
Mg2+ absolute requirement for divalent metal ions, best activator Escherichia coli
Mn2+ absolute requirement for divalent metal ions Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46600
-
2 * 46600 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + IMP + L-aspartate Acidithiobacillus ferrooxidans first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Pyrococcus sp. first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Haemophilus influenzae first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Bacillus subtilis first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Mus musculus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Escherichia coli first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Homo sapiens first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Rattus norvegicus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Triticum aestivum first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Oryctolagus cuniculus first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Zea mays first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Arabidopsis thaliana first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Dictyostelium discoideum first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Schizosaccharomyces pombe first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Leishmania donovani first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Azotobacter vinelandii first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Trypanosoma cruzi first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Methanocaldococcus jannaschii first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate Pyrococcus sp. ST700 first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle GDP + phosphate + adenylosuccinate
-
?

Organism

Organism UniProt Comment Textmining
Acidithiobacillus ferrooxidans
-
-
-
Arabidopsis thaliana
-
-
-
Azotobacter vinelandii
-
-
-
Bacillus subtilis
-
-
-
Dictyostelium discoideum
-
-
-
Escherichia coli
-
-
-
Haemophilus influenzae
-
-
-
Homo sapiens
-
human
-
Leishmania donovani
-
-
-
Methanocaldococcus jannaschii
-
-
-
Mus musculus
-
mouse
-
Oryctolagus cuniculus
-
rabbit
-
Pyrococcus sp.
-
-
-
Pyrococcus sp. ST700
-
-
-
Rattus norvegicus
-
rat
-
Schizosaccharomyces pombe
-
-
-
Triticum aestivum
-
wheat
-
Trypanosoma cruzi
-
-
-
Zea mays
-
maize
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus
-
Oryctolagus cuniculus
-
Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-aspartate
-
Acidithiobacillus ferrooxidans GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Pyrococcus sp. GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Haemophilus influenzae GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Bacillus subtilis GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Mus musculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Escherichia coli GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Homo sapiens GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Rattus norvegicus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Triticum aestivum GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Oryctolagus cuniculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Zea mays GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Arabidopsis thaliana GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Dictyostelium discoideum GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Schizosaccharomyces pombe GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Leishmania donovani GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Azotobacter vinelandii GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Trypanosoma cruzi GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Methanocaldococcus jannaschii GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Acidithiobacillus ferrooxidans GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Pyrococcus sp. GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Haemophilus influenzae GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Bacillus subtilis GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Mus musculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Escherichia coli GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Homo sapiens GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Rattus norvegicus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Triticum aestivum GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Oryctolagus cuniculus GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Zea mays GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Arabidopsis thaliana GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Dictyostelium discoideum GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Schizosaccharomyces pombe GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Leishmania donovani GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Azotobacter vinelandii GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Trypanosoma cruzi GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Methanocaldococcus jannaschii GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate
-
Pyrococcus sp. ST700 GDP + phosphate + adenylosuccinate
-
?
GTP + IMP + L-aspartate first committed step in the de novo biosynthesis of adenosine monophosphate and component of the purine nucleotide cycle Pyrococcus sp. ST700 GDP + phosphate + adenylosuccinate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 46600 Escherichia coli

Synonyms

Synonyms Comment Organism
adenosylsuccinate synthetase
-
Escherichia coli
adenosylsuccinate synthetase
-
Rattus norvegicus
Adenylosuccinate synthetase
-
Acidithiobacillus ferrooxidans
Adenylosuccinate synthetase
-
Pyrococcus sp.
Adenylosuccinate synthetase
-
Haemophilus influenzae
Adenylosuccinate synthetase
-
Bacillus subtilis
Adenylosuccinate synthetase
-
Mus musculus
Adenylosuccinate synthetase
-
Homo sapiens
Adenylosuccinate synthetase
-
Triticum aestivum
Adenylosuccinate synthetase
-
Oryctolagus cuniculus
Adenylosuccinate synthetase
-
Zea mays
Adenylosuccinate synthetase
-
Arabidopsis thaliana
Adenylosuccinate synthetase
-
Dictyostelium discoideum
Adenylosuccinate synthetase
-
Schizosaccharomyces pombe
Adenylosuccinate synthetase
-
Leishmania donovani
Adenylosuccinate synthetase
-
Azotobacter vinelandii
Adenylosuccinate synthetase
-
Trypanosoma cruzi
Adenylosuccinate synthetase
-
Methanocaldococcus jannaschii
AMPSase
-
Acidithiobacillus ferrooxidans
AMPSase
-
Pyrococcus sp.
AMPSase
-
Haemophilus influenzae
AMPSase
-
Bacillus subtilis
AMPSase
-
Mus musculus
AMPSase
-
Escherichia coli
AMPSase
-
Homo sapiens
AMPSase
-
Rattus norvegicus
AMPSase
-
Triticum aestivum
AMPSase
-
Oryctolagus cuniculus
AMPSase
-
Zea mays
AMPSase
-
Arabidopsis thaliana
AMPSase
-
Dictyostelium discoideum
AMPSase
-
Schizosaccharomyces pombe
AMPSase
-
Leishmania donovani
AMPSase
-
Azotobacter vinelandii
AMPSase
-
Trypanosoma cruzi
AMPSase
-
Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4
-
GTP
-
Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus acidic isoenzyme
-
5.9
Rattus norvegicus basic isoenzyme
-
8.9
Mus musculus most abundant isozyme
-
8.9