Protein Variants | Comment | Organism |
---|---|---|
R303L | mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable | Escherichia coli |
R304L | mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable | Escherichia coli |
R305L | mutant enzymes R303L, R304L, and R305L exhibit a 50-200fold increase in their Km values for Asp relative to the wild-type enzyme. The Km values for GTP and IMP are comparable | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
GTP | Asp, mutant R305L | Escherichia coli | |
0.0201 | - |
GTP | mutant R303L | Escherichia coli | |
0.026 | - |
GTP | wild-type | Escherichia coli | |
0.0265 | - |
GTP | mutant R304L | Escherichia coli | |
0.0278 | - |
IMP | wild-type | Escherichia coli | |
0.0296 | - |
IMP | mutant R304L | Escherichia coli | |
0.0305 | - |
IMP | mutant R305L | Escherichia coli | |
0.0306 | - |
GTP | mutant R305L | Escherichia coli | |
0.0352 | - |
IMP | mutant R303L | Escherichia coli | |
0.057 | - |
Asp | mutant R303L | Escherichia coli | |
0.0657 | - |
Asp | mutant R303L | Escherichia coli | |
0.23 | - |
Asp | Asp, wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes R303L, R304L, and R305L | - |
Purification (Comment) | Organism |
---|---|
mutant enzymes R303L, R304L, and R305L | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |