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Literature summary for 6.3.4.3 extracted from

  • Celeste, L.R.; Chai, G.; Bielak, M.; Minor, W.; Lovelace, L.L.; Lebioda, L.
    Mechanism of N10-formyltetrahydrofolate synthetase derived from complexes with intermediates and inhibitors (2012), Protein Sci., 21, 219-228.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Y1 Moorella thermoacetica

Crystallization (Commentary)

Crystallization (Comment) Organism
native protein, folate complex, ADP-formylphosphate complex, and ZD9331-formylphosphate complex, hanging drop vapor diffusion method. High salt conditions contain 38-46% (w/v) saturated ammonium sulfate, 1 mM , and dithiohtreitol, 1-3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0-8.0). Low salt conditions contain 18-22% (w/v) PEG 6-8K, 0.2 M ammonium sulfate, 1 mM dithiothreitol, in 75 mM KMB pH 7.0-8.5 Moorella thermoacetica

Inhibitors

Inhibitors Comment Organism Structure
folate
-
Moorella thermoacetica
ZD9331
-
Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, X-ray crystallography Moorella thermoacetica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + formate + tetrahydrofolate Moorella thermoacetica
-
ADP + phosphate + 10-formyltetrahydrofolate
-
?

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica P21164
-
-

Purification (Commentary)

Purification (Comment) Organism
heparin agarose column chromatography and phenyl Sepharose column chromatography Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + formate + tetrahydrofolate
-
Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 60000, X-ray crystallography Moorella thermoacetica

Synonyms

Synonyms Comment Organism
FTHFS
-
Moorella thermoacetica
N10-formyltetrahydrofolate synthetase
-
Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
ATP
-
Moorella thermoacetica