Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Y1 | Moorella thermoacetica |
Crystallization (Comment) | Organism |
---|---|
native protein, folate complex, ADP-formylphosphate complex, and ZD9331-formylphosphate complex, hanging drop vapor diffusion method. High salt conditions contain 38-46% (w/v) saturated ammonium sulfate, 1 mM , and dithiohtreitol, 1-3.5% (w/v) PEG 1000 or PEG 1450, in 50-75 mM KMB (pH 7.0-8.0). Low salt conditions contain 18-22% (w/v) PEG 6-8K, 0.2 M ammonium sulfate, 1 mM dithiothreitol, in 75 mM KMB pH 7.0-8.5 | Moorella thermoacetica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
folate | - |
Moorella thermoacetica | |
ZD9331 | - |
Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, X-ray crystallography | Moorella thermoacetica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | Moorella thermoacetica | - |
ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | P21164 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin agarose column chromatography and phenyl Sepharose column chromatography | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + formate + tetrahydrofolate | - |
Moorella thermoacetica | ADP + phosphate + 10-formyltetrahydrofolate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 60000, X-ray crystallography | Moorella thermoacetica |
Synonyms | Comment | Organism |
---|---|---|
FTHFS | - |
Moorella thermoacetica |
N10-formyltetrahydrofolate synthetase | - |
Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Moorella thermoacetica |