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Literature summary for 6.3.4.3 extracted from
- Disruption of the mthfd1 gene reveals a monofunctional 10-formyltetrahydrofolate synthetase in mammalian mitochondria (2005), J. Biol. Chem., 280, 7597-7602.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a DCS disruption knockout mutant fibroblast SF6 cell line lacking the cytoplasmic isozyme but expressing active mitochondrial isozyme from a disruption mutant embryonic stem cell line | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | trifunctional cytosolic isozyme | Mus musculus | 5829 | - |
| mitochondrion | mitochondrial monofunctional isozyme | Mus musculus | 5739 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | Mus musculus | flow of one-carbon units through cytoplasmic and mitochondrial folate pathways, overview | ADP + phosphate + 10-formyltetrahydrofolate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q922D8 | DCS, complete gene and exon 27; gene mthfd1 encodes the cytosolic isozyme which is a trifunctional methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase enzyme DCS, a mitochondrial monofunctional 10-formyltetrahydrofolate synthetase isozyme is encoded by a different gene | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Mus musculus | - |
| embryonic stem cell | - |
Mus musculus | - |
| fibroblast | - |
Mus musculus | - |
| fibroblast | SF6 cell, immortalized fibroblast with disrupted gene encoding the trifunctional cytosolic DCS | Mus musculus | - |
| additional information | isozyme expression analysis and growth rates of cell lines, overview | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
mitochondrial isozyme activity in different cell lines, overview | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + formate + tetrahydrofolate | - |
Mus musculus | ADP + phosphate + 10-formyltetrahydrofolate | - |
r | |
| ATP + formate + tetrahydrofolate | flow of one-carbon units through cytoplasmic and mitochondrial folate pathways, overview | Mus musculus | ADP + phosphate + 10-formyltetrahydrofolate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 10-Formyltetrahydrofolate synthetase | - |
Mus musculus |
| 10-formylTHF synthetase | - |
Mus musculus |
| DCS | - |
Mus musculus |
| Formyltetrahydrofolate synthetase | - |
Mus musculus |
| methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase enzyme | - |
Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Mus musculus | |
| ATP | - |
Mus musculus | |
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