Cloned (Comment) | Organism |
---|---|
expression of pncB gene on a multicopy pUC19 plasmid in Salmonella typhimurium | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
nicotinate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.022 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the coupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.29 | - |
nicotinate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pncB gene, in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
pncB gene, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
bacterial suspension disruption and centrifugation, chromatography on Sephadex-G, DEAE-Toyopearl, Cibacron Blue and Sephacryl gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability | Organism |
---|---|
4ºC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
t1/2: at least 2 months | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
NAPRTase | - |
- |
nicotinate phosphoribosyltransferase | - |
- |
nicotinic acid phosphoribosyltransferase | - |
- |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Salmonella enterica subsp. enterica serovar Typhimurium |