Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
first order activity loss in the presence of 1/200 trypsin/enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
sensitive to trypsin, partial proteolysis, 4fold increase in t1/2 for inactivation in the presence of 1 mM MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae | |
ATP | 3 mM, competitive inhibition of nicotinic acid mononucleotide formation in mutants enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
nicotinate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.33 | 0.4 | nicotinate | mutant enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.3 | 48 | 5-phospho-alpha-D-ribose 1-diphosphate | mutant enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.5 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45500 | - |
mutant enzyme, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
45529 | - |
1 * 45529, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
45540 | - |
SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
45550 | - |
mutant enzyme, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P39683 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Storage Stability | Organism |
---|---|
4ΒΊC, as 70% saturated ammonium sulfate suspensions in 200 mM phosphate buffer, pH 8, 10% glycerol and 5 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | negligible ATPase activity in the absence of nicotinic acid | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Saccharomyces cerevisiae | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | mutants in the His-219 residue do catalyze the slow formation of nicotinic acid mononucleotide in the absence of ATP similarly to the wild type enzymes | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45529, gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Salmonella enterica subsp. enterica serovar Typhimurium | |
ATP | 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed | Saccharomyces cerevisiae | |
ATP | the Vmax of nicotinic acid mononucleotide synthesis activity is stimulated 10fold | Salmonella enterica subsp. enterica serovar Typhimurium |