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Literature summary for 6.3.4.21 extracted from

  • Imsande, J.
    A cross-linked control system. I. Properties of a triphosphate-dependent nicotinic acid mononucleotide pyrophosphorylase from Bacillus subtilis (1964), Biochim. Biophys. Acta, 85, 255-264.
    View publication on PubMed
Search for more literature for 6.3.4.21

Activating Compound

Activating Compound Comment Organism Structure
tripolyphosphate activation, can replace ATP Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
3-acetylpyridine
-
 Bacillus subtilis
3-Pyridylcarbinol weak inhibitor Bacillus subtilis
3-Pyridylcarboxaldehyde
-
 Bacillus subtilis
5-Fluoronicotinic acid
-
 Bacillus subtilis
KCN
-
 Bacillus subtilis
Methyl 3-pyridylcarbinol weak inhibitor Bacillus subtilis
additional information no inhibition by nicotinamide; no inhibition by quinolinic acid, AMP, GMP Bacillus subtilis
NAD+ not Bacillus subtilis
NADP+ not Bacillus subtilis
nicotinate mononucleotide not Bacillus subtilis
nucleoside diphosphates e.g. ADP or GDP Bacillus subtilis
Urea competitive to nicotinate mononucleotide Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008 0.0015 nicotinate
-
 Bacillus subtilis
0.06 0.1 5-phospho-alpha-D-ribose 1-diphosphate
-
 Bacillus subtilis
0.34
-
 GTP
-
 Bacillus subtilis
0.35
-
 ATP
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 SB19
-
Bacillus subtilis SB19
-
 SB19
-

Purification (Commentary)

Purification (Comment) Organism
partial, extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose, 60fold purified Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information one mol ATP is cleaved per mol product formed Bacillus subtilis ?
-
 ?
additional information one mol ATP is cleaved per mol product formed Bacillus subtilis SB19 ?
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O favoured reaction Bacillus subtilis beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O favoured reaction Bacillus subtilis SB19 beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
 r
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
-
 Bacillus subtilis beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
 ?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + GTP + H2O
-
 Bacillus subtilis SB19 beta-nicotinate D-ribonucleotide + diphosphate + GDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
nicotinic acid mononucleotide pyrophosphorylase
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP 1 mol ATP is hydrolyzed to ADP plus phosphate per mol product formed Bacillus subtilis
CTP activation, about 70% as effective as ATP Bacillus subtilis
GTP activation, equally effective as ATP Bacillus subtilis
ITP activation, about 90% as effective as ATP Bacillus subtilis
TTP activation, about 30% as effective as ATP Bacillus subtilis
UTP activation, about 50% as effective as ATP Bacillus subtilis