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Literature summary for 6.3.4.21 extracted from

  • Hayakawa, T.; Shibata, K.; Iwai, K.
    Purification and some properties of nicotinate phosphoribosyltransferase from hog liver (1984), Agric. Biol. Chem., 48, 445-453.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by ADP Sus scrofa
additional information no activation by AMP, 2'-AMP Sus scrofa
additional information no activation by 3'-AMP, 5'-AMP Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
ATP in the presence of Mn2+ Rattus norvegicus
ATP in the presence of Mn2+ Sus scrofa
GDP
-
Sus scrofa
GMP
-
Sus scrofa
IDP
-
Sus scrofa
IMP
-
Sus scrofa
ITP
-
Sus scrofa
Monoiodoacetic acid 1 mM and above Sus scrofa
additional information no inhibition by antibodies against pig kidney quinolinate phosphoribosyltransferase; no inhibition by nicotinamide mononucleotide, deamino-NAD+ Sus scrofa
N-ethylmaleimide 0.1 mM and above Sus scrofa
NAD+ weak inhibitor Sus scrofa
NADH weak inhibitor Sus scrofa
NADP+ weak inhibitor Sus scrofa
NADPH weak inhibitor Sus scrofa
Nicotinate analogues not Sus scrofa
nicotinate mononucleotide competitive to nicotinate and noncompetitive to phosphoribobose diphosphate Sus scrofa
PCMB 0.01 mM and above Sus scrofa
Pyrazine 2-carboxylic acid
-
Sus scrofa
Pyridazine-4,5-dicarboxylic acid weak, 10% less activity Sus scrofa

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ requirement, slight synergism with ATP, more effective than Mg2+ with or without ATP Sus scrofa
Fe2+ slight stimulation, synergism with ATP Sus scrofa
Mg2+ requirement Sus scrofa
Mg2+ synergism with ATP Sus scrofa
Mn2+ requirement, 7.5 mM, no synergism with ATP, more effective than Mg2+ with or without ATP Sus scrofa
additional information absolute requirement of divalent cation, no activation with Ba2+, Ca2+, Cu2+, Cd2+, Al3+, Fe3+ Sus scrofa
Zn2+ slight stimulation Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 63000, SDS-PAGE Sus scrofa
120000
-
liver, SDS-PAGE Sus scrofa

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
male Wistar strain
-
Rattus norvegicus male Wistar
-
male Wistar strain
-
Sus scrofa
-
hog
-

Purification (Commentary)

Purification (Comment) Organism
ammonium hydroxide precipitation, chromatography on DEAE-Sephadex, Sephacryl and phenyl-Sepharose Sus scrofa
homogenates Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
blood clot
-
Rattus norvegicus
-
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
liver
-
Sus scrofa
-
additional information distribution in rat tissues: trace activities in brain, lung or stomach, not in thigh muscle, testis or serum Rattus norvegicus
-
pancreas
-
Rattus norvegicus
-
small intestine
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.095
-
crude extract Sus scrofa
160.5
-
DEAE-Sephadex fraction Sus scrofa
421.2
-
Sephacryl fraction Sus scrofa
457.3
-
phenyl-Sepharose fraction Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Rattus norvegicus beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine Sus scrofa beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Rattus norvegicus male Wistar beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
NPRTase
-
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI: 4.8 Sus scrofa
7.3 7.4
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
ATP activation Rattus norvegicus
ATP activation Sus scrofa
ATP together with Mg2+ Sus scrofa
ATP together with Co2+ Sus scrofa
GTP not Sus scrofa
ITP not Sus scrofa