Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by ADP | Sus scrofa | |
additional information | no activation by AMP, 2'-AMP | Sus scrofa | |
additional information | no activation by 3'-AMP, 5'-AMP | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | in the presence of Mn2+ | Rattus norvegicus | |
ATP | in the presence of Mn2+ | Sus scrofa | |
GDP | - |
Sus scrofa | |
GMP | - |
Sus scrofa | |
IDP | - |
Sus scrofa | |
IMP | - |
Sus scrofa | |
ITP | - |
Sus scrofa | |
Monoiodoacetic acid | 1 mM and above | Sus scrofa | |
additional information | no inhibition by antibodies against pig kidney quinolinate phosphoribosyltransferase; no inhibition by nicotinamide mononucleotide, deamino-NAD+ | Sus scrofa | |
N-ethylmaleimide | 0.1 mM and above | Sus scrofa | |
NAD+ | weak inhibitor | Sus scrofa | |
NADH | weak inhibitor | Sus scrofa | |
NADP+ | weak inhibitor | Sus scrofa | |
NADPH | weak inhibitor | Sus scrofa | |
Nicotinate analogues | not | Sus scrofa | |
nicotinate mononucleotide | competitive to nicotinate and noncompetitive to phosphoribobose diphosphate | Sus scrofa | |
PCMB | 0.01 mM and above | Sus scrofa | |
Pyrazine 2-carboxylic acid | - |
Sus scrofa | |
Pyridazine-4,5-dicarboxylic acid | weak, 10% less activity | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | requirement, slight synergism with ATP, more effective than Mg2+ with or without ATP | Sus scrofa | |
Fe2+ | slight stimulation, synergism with ATP | Sus scrofa | |
Mg2+ | requirement | Sus scrofa | |
Mg2+ | synergism with ATP | Sus scrofa | |
Mn2+ | requirement, 7.5 mM, no synergism with ATP, more effective than Mg2+ with or without ATP | Sus scrofa | |
additional information | absolute requirement of divalent cation, no activation with Ba2+, Ca2+, Cu2+, Cd2+, Al3+, Fe3+ | Sus scrofa | |
Zn2+ | slight stimulation | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
2 * 63000, SDS-PAGE | Sus scrofa |
120000 | - |
liver, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar strain | - |
Rattus norvegicus male Wistar | - |
male Wistar strain | - |
Sus scrofa | - |
hog | - |
Purification (Comment) | Organism |
---|---|
ammonium hydroxide precipitation, chromatography on DEAE-Sephadex, Sephacryl and phenyl-Sepharose | Sus scrofa |
homogenates | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
blood clot | - |
Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Sus scrofa | - |
additional information | distribution in rat tissues: trace activities in brain, lung or stomach, not in thigh muscle, testis or serum | Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
small intestine | - |
Rattus norvegicus | - |
spleen | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.095 | - |
crude extract | Sus scrofa |
160.5 | - |
DEAE-Sephadex fraction | Sus scrofa |
421.2 | - |
Sephacryl fraction | Sus scrofa |
457.3 | - |
phenyl-Sepharose fraction | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Rattus norvegicus | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | no phosphoribosylation of nicotinamide, quinolic acid, adenine or hypoxanthine | Sus scrofa | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Rattus norvegicus male Wistar | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 63000, SDS-PAGE | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
NPRTase | - |
- |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 4.8 | Sus scrofa |
7.3 | 7.4 | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Rattus norvegicus | |
ATP | activation | Sus scrofa | |
ATP | together with Mg2+ | Sus scrofa | |
ATP | together with Co2+ | Sus scrofa | |
GTP | not | Sus scrofa | |
ITP | not | Sus scrofa |