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Literature summary for 6.3.4.21 extracted from

  • Imsande, J.; Handler, P.
    Biosynthesis of diphosphopyridine nucleotide. III. Nicotinic acid mononucleotide pyrophosphorylase (1961), J. Biol. Chem., 236, 525-530.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by ADP Bos taurus
additional information 3,5-cycloadenosine, borate and sulfate do not affect the reaction rate Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
Bos taurus
Mg2+ at higher concentrations than ATP, when free Mg2+ ions occur Bos taurus
additional information no inhibition by nicotinamide; no inhibition by nicotinate, up to 10 mM Bos taurus
nicotinate mononucleotide competitive to both substrates Bos taurus
tripolyphosphate
-
Bos taurus
UTP weak Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
nicotinate
-
Bos taurus
0.05
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bos taurus
Mg2+ synergism with ATP Bos taurus
MgATP2- maximal activation at equimolar levels of Mg2+ and ATP, presumably enzyme structure stabilizing Bos taurus
additional information no activation by borate, sulfate Bos taurus
phosphate
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O Bos taurus
-
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
beef
-

Purification (Commentary)

Purification (Comment) Organism
extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
-
Bos taurus

Storage Stability

Storage Stability Organism
-12°C, partially purified enzyme preparation, t1/2: at least 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Bos taurus beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
?
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O
-
Bos taurus beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
-
r

Synonyms

Synonyms Comment Organism
nicotinic acid mononucleotide pyrophosphorylase
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
broad, phosphorolysis Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
active over a wide range Bos taurus
5.5 8 about half-maximal activity at pH 5.5 and about 85% of maximal activity at pH 8 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
inactivation below, phosphorolysis Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP activation Bos taurus
ATP 60-70% stimulation with different enzyme preparations, the largest effects are observed with aged preparations of the enzyme Bos taurus
ATP together with Mg2+ Bos taurus
CTP 20-30% as effective as ATP Bos taurus
GTP 20-30% as effective as ATP Bos taurus
ITP 20-30% as effective as ATP Bos taurus