Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by ADP | Bos taurus | |
additional information | 3,5-cycloadenosine, borate and sulfate do not affect the reaction rate | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Bos taurus | |
Mg2+ | at higher concentrations than ATP, when free Mg2+ ions occur | Bos taurus | |
additional information | no inhibition by nicotinamide; no inhibition by nicotinate, up to 10 mM | Bos taurus | |
nicotinate mononucleotide | competitive to both substrates | Bos taurus | |
tripolyphosphate | - |
Bos taurus | |
UTP | weak | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
nicotinate | - |
Bos taurus | |
0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Bos taurus | |
Mg2+ | synergism with ATP | Bos taurus | |
MgATP2- | maximal activation at equimolar levels of Mg2+ and ATP, presumably enzyme structure stabilizing | Bos taurus | |
additional information | no activation by borate, sulfate | Bos taurus | |
phosphate | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | Bos taurus | - |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
beef | - |
Purification (Comment) | Organism |
---|---|
extraction, ammonium sulfate fractionation and chromatography on DEAE-cellulose | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-12°C, partially purified enzyme preparation, t1/2: at least 3 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Bos taurus | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Bos taurus | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
nicotinic acid mononucleotide pyrophosphorylase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
broad, phosphorolysis | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
active over a wide range | Bos taurus |
5.5 | 8 | about half-maximal activity at pH 5.5 and about 85% of maximal activity at pH 8 | Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
inactivation below, phosphorolysis | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | activation | Bos taurus | |
ATP | 60-70% stimulation with different enzyme preparations, the largest effects are observed with aged preparations of the enzyme | Bos taurus | |
ATP | together with Mg2+ | Bos taurus | |
CTP | 20-30% as effective as ATP | Bos taurus | |
GTP | 20-30% as effective as ATP | Bos taurus | |
ITP | 20-30% as effective as ATP | Bos taurus |