Any feedback?
Literature summary for 6.3.4.21 extracted from
- Pathway of diphosphopyridine nucleotide biosynthesis in Escherichia coli (1961), J. Biol. Chem., 236, 1494-1497.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GSH | activation | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
nicotinate | - |
Escherichia coli | |
| 0.03 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | |
| 0.4 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Escherichia coli | |
| Mg2+ | maximal activity: 20 mM | Escherichia coli | |
| Mg2+ | synergism with ATP | Escherichia coli | |
| NaF | activation | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | Escherichia coli | - |
beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| bacteria disruption by alumina grinding, protamine sulfate precipitation and chromatography on DEAE-cellulose | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Escherichia coli | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
? | |
| nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | - |
Escherichia coli | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinic acid mononucleotide pyrophosphorylase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
active over a wide range | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | activation | Escherichia coli | |
| ATP | together with Mg2+ | Escherichia coli | |
| UTP | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
