Cloned (Comment) | Organism |
---|---|
human CTP synthetase 1 is expressed and purified from a Saccharomyces cerevisiae ura7delta ura8delta double mutant that lacks CTP synthetase activity | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T455A | T455A mutation causes a 78% decrease in protein kinase A phosphorylation | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | assay with 10 mM MgCl2 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His6-tagged human CTP synthetase 1 is purified by loading onto a Ni2+-NTA column | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
CTP synthetase 1 activity of the T455A mutant enzyme is 2fold higher than the wild type enzyme. T455A mutation causes a 44% decrease in the amount of human CTP synthetase 1 that is phosphorylated in Saccharomyces cerevisiae cells, accompanied by a 2.5fold increase in the cellular concentration of CTP and a 1.5-fold increase in the choline-dependent synthesis of phosphatidylcholine | Homo sapiens |
additional information | - |
Thr 455 is identified as a major site of phosphorylation by protein kinase A, phosphorylation at Thr455 results in the inhibition of activity in vitro and in vivo. Data indicate that phosphorylation at Thr455 attenuates the choline-dependent synthesis of phosphatidylcholine when CTP synthetase 1 enzyme is expressed in Saccharomyces cerevisiae | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UTP + Gln + H2O | - |
Homo sapiens | ADP + CTP + Glu + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CTP synthetase 1 | - |
Homo sapiens |
CTPS1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |