Literature summary for 6.3.4.2 extracted from

Kursula, P.; Flodin, S.; Ehn, M.; Hammarstroem, M.; Schueler, H.; Nordlund, P.; Stenmark, P.
Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy (2006), Acta Crystallogr. Sect. F, 62, 613-617.

Application

Application	Comment	Organism
drug development	structure serves as a basis for structure-based design of anti-proliferative inhibitors	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
cDNA encoding the synthetase domain of human CTPS (isoform 1) is subcloned into the pNIC-Bsa4 vector, the resulting construct codes for a protein with an N-terminal hexahistidine tag with an integrated TEV protease-cleavage site, the entire plasmid is transformed in Escherichia coli BL-21	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure analysis discloses a homotetrameric structure and each active site is formed by three different subunits. Sulfate ions bound to the active sites indicate the positions of phosphate-binding sites for the substrates ATP and UTP and the feedback inhibitor CTP. Together with earlier structures of bacterial CTPS, the human CTPS structure provides an extended understanding of the structurefunction relationship of CTPS-family members	Homo sapiens
the protein is crystallized by the hanging-drop method, equilibrating 1 ml protein solution and 1 ml of a solution containing 0.1 M Tris pH 8.8, 1.2 M (NH4)2SO4 and 50 mM malonic acid against 0.5 ml of a well solution containing 0.1 M Tris pH 8.8 and 1.2 M (NH4)2SO4. Crystals with a maximum dimension of 0.03 nm formed in 3 d	Homo sapiens

Crystallization (Comment)

Organism

crystal structure analysis discloses a homotetrameric structure and each active site is formed by three different subunits. Sulfate ions bound to the active sites indicate the positions of phosphate-binding sites for the substrates ATP and UTP and the feedback inhibitor CTP. Together with earlier structures of bacterial CTPS, the human CTPS structure provides an extended understanding of the structurefunction relationship of CTPS-family members

Homo sapiens

the protein is crystallized by the hanging-drop method, equilibrating 1 ml protein solution and 1 ml of a solution containing 0.1 M Tris pH 8.8, 1.2 M (NH4)2SO4 and 50 mM malonic acid against 0.5 ml of a well solution containing 0.1 M Tris pH 8.8 and 1.2 M (NH4)2SO4. Crystals with a maximum dimension of 0.03 nm formed in 3 d

Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
protein is purified by using HisTrap HP and Superdex 75 columns	Homo sapiens

Synonyms

Synonyms	Comment	Organism
CTS	-	Homo sapiens
cytidine triphosphate synthetase	-	Homo sapiens