Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(5R)-5-hydroxy-L-lysine | - |
Escherichia coli | |
4,5-trans-dehydro-L-lysine | - |
Escherichia coli | |
8-azaadenosine 5-triphosphate | - |
Escherichia coli | |
adenosine 5-O-(1-thiotriphosphate) | - |
Escherichia coli | |
alpha,beta-methyleneadenosine 5'-triphosphate | - |
Escherichia coli | |
aminoethylcysteine | - |
Escherichia coli | |
aminoethylserine | - |
Escherichia coli | |
benzimidazoleriboside 5-triphosphate | - |
Escherichia coli | |
beta,gamma-methyleneadenosine 5'-triphosphate | - |
Escherichia coli | |
cadaverine | - |
Escherichia coli | |
ethanolamine | - |
Escherichia coli | |
histamine | - |
Escherichia coli | |
L-lysinamide | - |
Escherichia coli | |
L-ornithine | - |
Escherichia coli | |
mutated tRNAIle2 | C34G-tRNAILe2 | Escherichia coli | |
N-acetylethylenediamine | - |
Escherichia coli | |
N-alpha-methyl-L-lysine | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
ATP | pH 8.5, 21°C | Escherichia coli | |
0.0018 | - |
7-deazaadenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli | |
0.01 | - |
8-azidoadenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli | |
0.0217 | - |
N6-methyladenosine-5-triphosphate | pH 8.5, 21°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | Escherichia coli | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P52097 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrate: N1-methyladenosine-5'-triphosphate, 2-aminoadenosine-5'-triphosphate, 2-amino-6-chloropurineriboside-5'-triphosphate, and 6-chloropurineriboside-5'-triphosphate | Escherichia coli | ? | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + 7-deazaadenosine 5'-triphosphate | Vmax/Km is 8.6% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + 7-deazaadenosine 5'-phosphate + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + 8-azidoadenosine 5'-triphosphate | Vmax/Km is 2.3% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + 8-azidoadenosine 5'-phosphate + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + ATP | - |
Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + ATP | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + N6-methyladenosine-5'-triphosphate | Vmax/Km is 4.7% of Vmax/Km for ATP | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + N6-methyladenosine-5'-phosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tRNAIle-lysidine synthetase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9.5 | - |
Escherichia coli |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000016 | - |
pH 8.5, 21°C | Escherichia coli | adenosine-5-O-(1-thiotriphosphate) | |
0.000035 | - |
pH 8.5, 21°C | Escherichia coli | alpha,beta-methylene-ATP | |
0.000045 | - |
pH 8.5, 21°C | Escherichia coli | aminoethylcysteine | |
0.000082 | - |
pH 8.5, 21°C | Escherichia coli | beta,gamma-methyleneadenosine 5'-triphosphate | |
0.000085 | - |
pH 8.5, 21°C, C34G-tRNAILe2 | Escherichia coli | mutated tRNAIle2 | |
0.0004 | - |
pH 8.5, 21°C | Escherichia coli | aminoethylserine | |
0.0025 | - |
pH 8.5, 21°C | Escherichia coli | 4,5-trans-dehydrolysine | |
0.0068 | - |
pH 8.5, 21°C | Escherichia coli | N-acetylethylenediamine | |
0.0176 | - |
pH 8.5, 21°C | Escherichia coli | (5R)-5-hydroxylysine | |
0.105 | - |
pH 8.5, 21°C | Escherichia coli | benzimidazoleriboside-5-triphosphate | |
0.255 | - |
pH 8.5, 21°C | Escherichia coli | 8-azaadenosine-5-triphosphate | |
0.33 | - |
pH 8.5, 21°C | Escherichia coli | histamine | |
0.62 | - |
pH 8.5, 21°C | Escherichia coli | lysinamide | |
0.7 | - |
pH 8.5, 21°C | Escherichia coli | ornithine | |
0.91 | - |
pH 8.5, 21°C | Escherichia coli | cadaverine | |
1.3 | - |
pH 8.5, 21°C | Escherichia coli | N-alpha-methyl-lysine | |
1.77 | - |
pH 8.5, 21°C | Escherichia coli | ethanolamine |
General Information | Comment | Organism |
---|---|---|
physiological function | bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase | Escherichia coli |