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Literature summary for 6.3.4.19 extracted from

  • Salowe, S.P.; Wiltsie, J.; Hawkins, J.C.; Sonatore, L.M.
    The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase (2009), J. Biol. Chem., 284, 9656-9662.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(5R)-5-hydroxy-L-lysine
-
Escherichia coli
4,5-trans-dehydro-L-lysine
-
Escherichia coli
8-azaadenosine 5’-triphosphate
-
Escherichia coli
adenosine 5’-O-(1-thiotriphosphate)
-
Escherichia coli
alpha,beta-methyleneadenosine 5'-triphosphate
-
Escherichia coli
aminoethylcysteine
-
Escherichia coli
aminoethylserine
-
Escherichia coli
benzimidazoleriboside 5’-triphosphate
-
Escherichia coli
beta,gamma-methyleneadenosine 5'-triphosphate
-
Escherichia coli
cadaverine
-
Escherichia coli
ethanolamine
-
Escherichia coli
histamine
-
Escherichia coli
L-lysinamide
-
Escherichia coli
L-ornithine
-
Escherichia coli
mutated tRNAIle2 C34G-tRNAILe2 Escherichia coli
N-acetylethylenediamine
-
Escherichia coli
N-alpha-methyl-L-lysine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
ATP pH 8.5, 21°C Escherichia coli
0.0018
-
7-deazaadenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli
0.01
-
8-azidoadenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli
0.0217
-
N6-methyladenosine-5’-triphosphate pH 8.5, 21°C Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[tRNAIle2]-cytidine34 + L-lysine + ATP Escherichia coli bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P52097
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: N1-methyladenosine-5'-triphosphate, 2-aminoadenosine-5'-triphosphate, 2-amino-6-chloropurineriboside-5'-triphosphate, and 6-chloropurineriboside-5'-triphosphate Escherichia coli ?
-
?
[tRNAIle2]-cytidine34 + L-lysine + 7-deazaadenosine 5'-triphosphate Vmax/Km is 8.6% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + 7-deazaadenosine 5'-phosphate + diphosphate
-
?
[tRNAIle2]-cytidine34 + L-lysine + 8-azidoadenosine 5'-triphosphate Vmax/Km is 2.3% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + 8-azidoadenosine 5'-phosphate + diphosphate
-
?
[tRNAIle2]-cytidine34 + L-lysine + ATP
-
Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?
[tRNAIle2]-cytidine34 + L-lysine + ATP bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate
-
?
[tRNAIle2]-cytidine34 + L-lysine + N6-methyladenosine-5'-triphosphate Vmax/Km is 4.7% of Vmax/Km for ATP Escherichia coli [tRNAIle2]-2-L-lysylcytidine34 + N6-methyladenosine-5'-phosphate + diphosphate
-
?

Synonyms

Synonyms Comment Organism
tRNAIle-lysidine synthetase
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9.5
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000016
-
pH 8.5, 21°C Escherichia coli adenosine-5’-O-(1-thiotriphosphate)
0.000035
-
pH 8.5, 21°C Escherichia coli alpha,beta-methylene-ATP
0.000045
-
pH 8.5, 21°C Escherichia coli aminoethylcysteine
0.000082
-
pH 8.5, 21°C Escherichia coli beta,gamma-methyleneadenosine 5'-triphosphate
0.000085
-
pH 8.5, 21°C, C34G-tRNAILe2 Escherichia coli mutated tRNAIle2
0.0004
-
pH 8.5, 21°C Escherichia coli aminoethylserine
0.0025
-
pH 8.5, 21°C Escherichia coli 4,5-trans-dehydrolysine
0.0068
-
pH 8.5, 21°C Escherichia coli N-acetylethylenediamine
0.0176
-
pH 8.5, 21°C Escherichia coli (5R)-5-hydroxylysine
0.105
-
pH 8.5, 21°C Escherichia coli benzimidazoleriboside-5’-triphosphate
0.255
-
pH 8.5, 21°C Escherichia coli 8-azaadenosine-5’-triphosphate
0.33
-
pH 8.5, 21°C Escherichia coli histamine
0.62
-
pH 8.5, 21°C Escherichia coli lysinamide
0.7
-
pH 8.5, 21°C Escherichia coli ornithine
0.91
-
pH 8.5, 21°C Escherichia coli cadaverine
1.3
-
pH 8.5, 21°C Escherichia coli N-alpha-methyl-lysine
1.77
-
pH 8.5, 21°C Escherichia coli ethanolamine

General Information

General Information Comment Organism
physiological function bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase Escherichia coli