Literature summary for 6.3.4.16 extracted from

Wang, Y.; Chang, L.; Zhai, J.; Wu, Q.; Wang, D.; Wang, Y.
Generation of carbamoyl phosphate synthetase 1 reporter cell lines for the assessment of ammonia metabolism (2017), J. Cell. Mol. Med., 21, 3214-3223 .

Application

Application	Comment	Organism
analysis	development of a CPS1-reporter system for the assessment of ammonia metabolism. Labeling of CPS1 gene in cell lines HepG2, and LO2, with fluorescence protein. Cellular detoxification enhancers are selected among a collection of 182 small molecules. In both CPS1 reporter cell lines, the fluorescence intensity is positively correlated with cellular CPS1 mRNA expression, ammonia elimination and secreted urea, and reflects ammonia detoxification in a dose-dependent manner	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of CPS1 in the absence and in the presence of N-acetyl-L-glutamate. N-acetyl-L-glutamate binds at the C-terminal domain of CPS1 and triggers long-range conformational changes affecting the two distant phosphorylation domains. The changes, concerted with the binding of nucleotides, result in a remodeling that stabilizes the catalytically competent conformation and the building of an about 35 A long tunnel that allows migration of the carbamate intermediate to the second phosphorylation site, where carbamoyl phosphate is produced	Homo sapiens

Crystallization (Comment)

Organism

structures of CPS1 in the absence and in the presence of N-acetyl-L-glutamate. N-acetyl-L-glutamate binds at the C-terminal domain of CPS1 and triggers long-range conformational changes affecting the two distant phosphorylation domains. The changes, concerted with the binding of nucleotides, result in a remodeling that stabilizes the catalytically competent conformation and the building of an about 35 A long tunnel that allows migration of the carbamate intermediate to the second phosphorylation site, where carbamoyl phosphate is produced

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	P31327	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
Hep-G2 cell	-	Homo sapiens	-
LO-2 cell	-	Homo sapiens	-

Synonyms

Synonyms	Comment	Organism
CPS1	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	labeling of CPS1 gene in cell lines HepG2, and LO2, with fluorescence protein. In both CPS1 reporter cell lines, the fluorescence intensity is positively correlated with cellular CPS1 mRNA expression, ammonia elimination and secreted urea, and reflects ammonia detoxification in a dose-dependent manner. High-level CPS1 reporter clones also reserve other critical hepatocellular functions, for example albumin secretion and cytochrome 450 metabolic functions. Sodium phenylbutyrate and resveratrol enhance metabolism-related gene expression and liver-enriched transcription factors C/EBPalpha, HNF4alpha	Homo sapiens