General Stability | Organism |
---|---|
stabilty under pressure, progressive inactivation down to 50% of initial activity at 200 MPa, this residual activity is constant at 200 MPa for at least 50 min | Pyrococcus abyssi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 1 mM, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
CTP | 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
dCTP | 1 mM, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
dGTP | 1 mM, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
ITP | 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
TTP | 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
UTP | 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi | |
XTP | 0.3 mM, about 50% inhibition, inhibition is more pronounced at 37°C compared to 70°C | Pyrococcus abyssi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
CO2 | NaHCO3, 37°C, pH 8 | Pyrococcus abyssi | |
7.2 | - |
CO2 | NaHCO3, 70°C, pH 7.5 | Pyrococcus abyssi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46600 | - |
1 * 46600, SDS-PAGE | Pyrococcus abyssi |
50700 | - |
gel filtration | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Storage Stability | Organism |
---|---|
4°C, 6 months, stable | Pyrococcus abyssi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + NH3 + CO2 + H2O | - |
Pyrococcus abyssi | ADP + phosphate + carbamoylphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 46600, SDS-PAGE | Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
carbamoyl-phosphate synthetase | - |
Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
3 h, 50% loss of activity | Pyrococcus abyssi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
70°C | Pyrococcus abyssi |
7.6 | 8.6 | at 37°C | Pyrococcus abyssi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.7 | 8.5 | pH 5.7: about 60% of maximal activit, pH 8.5: about 80% of maximal activity, 70°C | Pyrococcus abyssi |
7 | 10 | pH 7.0: about 60% of maximal activity, pH 10.0: about 60% of maximal activity, 37°C | Pyrococcus abyssi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pyrococcus abyssi | isoelectric focusing | - |
4.7 |