Literature summary for 6.3.4.16 extracted from

Purcarea, C.; Herve, G.; Cunin, R.; Evans, D.R.
Cloning, expression, and structure analysis of carbamate kinase-like carbamoyl phosphate synthetase from Pyrococcus abyssi (2001), Extremophiles, 5, 229-239.

Search for more literature for 6.3.4.16

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli and Schizosaccharomyces pombe	Pyrococcus abyssi

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of deletion mutant lacking the last 13 residues of the carboxyl end, 70% decrease of the enzyme activity in comparison of the full-length protein	Pyrococcus abyssi

Inhibitors

Inhibitors	Comment	Organism	Structure
cytidine triphosphate	-	Pyrococcus abyssi

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0372	-	ATP	pH 8.0, deletion mutant lacking the last 13 residues of the carboxyl end	Pyrococcus abyssi
0.06	-	ATP	pH 8.0, recombinant enzyme	Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34360	-	-	Pyrococcus abyssi

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	Q9UWF8	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.014	-	recombinant enzyme expressed in Schizosaccharomyces pombe	Pyrococcus abyssi
0.12	-	deletion mutant lacking the last 13 residues of the carboxyl end	Pyrococcus abyssi
0.4	-	recombinant enzyme expressed in Escherischia coli	Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + carbamate	-	Pyrococcus abyssi	ADP + carbamoyl phosphate	-	r
ATP + NH3 + CO2 + H2O	three step reaction	Pyrococcus abyssi	ADP + phosphate + carbamoyl phosphate	-	ir

Synonyms

Synonyms	Comment	Organism
carbamate kinase-like carbamoyl phosphate synthetase	this enzyme is a intermediary form between carbamate kinase and carbamoyl phosphate synthetase	Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
additional information	-	grows optimally at temperatures around 100°C	Pyrococcus abyssi
90	-	-	Pyrococcus abyssi

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Release 2023.1 (January 2023)