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Literature summary for 6.3.4.16 extracted from
- Carbamoyl-phosphate synthase in Phycomyces blakesleeanus (1988), Biochim. Biophys. Acta, 955, 175-186.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetylglutamate | not required as activator | Phycomyces blakesleeanus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | - |
Phycomyces blakesleeanus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.93 | - |
ATP | - |
Phycomyces blakesleeanus | |
| 6.34 | - |
NH4+ | - |
Phycomyces blakesleeanus | |
| 10.5 | - |
HCO3- | - |
Phycomyces blakesleeanus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | free Mg2+ required for maximal activity | Phycomyces blakesleeanus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
| 130000 | - |
1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
| 188000 | - |
gel filtration | Phycomyces blakesleeanus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phycomyces blakesleeanus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phycomyces blakesleeanus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Phycomyces blakesleeanus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.87 | - |
- |
Phycomyces blakesleeanus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + NH4+ + HCO3- | - |
Phycomyces blakesleeanus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH4+ + HCO3- | ATP in form of MgATP2- | Phycomyces blakesleeanus | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| ATP + NH4+ + CO2 + H2O | no activity with Gln | Phycomyces blakesleeanus | ADP + phosphate + carbamoylphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 130000 + 1 * 55000, SDS-PAGE | Phycomyces blakesleeanus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 38.5 | - |
transition temperature | Phycomyces blakesleeanus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.8 | - |
Phycomyces blakesleeanus |
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