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Literature summary for 6.3.4.16 extracted from
- Human carbamylphosphate synthetase I. Stabilization, purification, and partial characterization of the enzyme from human liver (1980), J. Biol. Chem., 255, 7891-7895.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetylglutamate | activates | Homo sapiens |  |
| acetylglutamate | Km: 0.1 mM | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | above 20 mM | Homo sapiens | |
| Mn2+ | at or above 5 mM | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
NH4+ | - |
Homo sapiens | |
| 1.1 | - |
ATP | - |
Homo sapiens | |
| 6.7 | - |
HCO3- | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement for free Mg2+ in addition to the MgATP2- complex, inhibits above 20 mM | Homo sapiens | |
| Mn2+ | can substitute for Mg2+ in activation, more effective on a molar basis, free Mn2+ at 5 mM and above inhibits | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 165000 | - |
1 * 165000, SDS-PAGE | Homo sapiens |
| 190000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ATP + NH4+ + HCO3- | - |
Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| 2 ATP + NH4+ + HCO3- | ATP in form of MgATP2- | Homo sapiens | 2 ADP + phosphate + carbamoyl phosphate | - |
? | |
| ATP + NH4+ + CO2 + H2O | - |
Homo sapiens | ADP + phosphate + carbamoylphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 165000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | 50% of maximal activity at pH 7.0 and 8.5 | Homo sapiens |
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