Literature summary for 6.3.4.13 extracted from

Sampei, G.; Baba, S.; Kanagawa, M.; Yanai, H.; Ishii, T.; Kawai, H.; Fukai, Y.; Ebihara, A.; Nakagawa, N.; Kawai, G.
Crystal structures of glycinamide ribonucleotide synthetase, PurD, from thermophilic eubacteria (2010), J. Biochem., 148, 429-438.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermus thermophilus
expression in Escherichia coli	Geobacillus kaustophilus
expression in Escherichia coli	Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment)	Organism
native enzyme and in complex with ATP, to 2.4 and 1.8 A resolution, respectively. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syns and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP	Aquifex aeolicus
native enzyme and in complex with phosphate, with AMP and phosphate, and with AMP and glycine, to 2.21, 2.20, 1.9 and 2.21 A resolution, respectively. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syn's and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP	Geobacillus kaustophilus
selenomethionine derivative, to 2.8 A resolution. Comparison of crystal structures of GAR-syn from Thermus thermophilus, Geobacillus kaustophilus and Aquifex aeolicus. The orientations of the B domains are varied among GAR-syns and the molecular dynamics simulation suggests the mobility of the B domain. The B loop in the B domain fixes the position of the beta- and gamma-phosphate groups of the bound ATP	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	gel filtration	Thermus thermophilus
50000	-	gel filtration	Geobacillus kaustophilus

Organism

Organism	UniProt	Comment	Textmining
Aquifex aeolicus	O66949	-	-
Geobacillus kaustophilus	Q5L3C7	-	-
Thermus thermophilus	Q5SK40	-	-

Subunits

Subunits	Comment	Organism
monomer	1 * 45000, SDS-PAGE	Thermus thermophilus
monomer	1 * 50000, SDS-PAGE	Geobacillus kaustophilus

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Release 2023.1 (January 2023)