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Literature summary for 6.3.4.13 extracted from

  • Bera, A.K.; Chen, S.; Smith, J.L.; Zalkin, H.
    Temperature-dependent function of the glutamine phosphoribosylpyrophosphate amidotransferase ammonia channel and coupling with glycinamide ribonucleotide synthetase in a hyperthermophile (2000), J. Bacteriol., 182, 3734-3739.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.13

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.59
-
 glycine pH 7.9, 90°C, with Mg2+ Aquifex aeolicus
1.01
-
 ATP pH 7.9, 90°C, with Mg2+ Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
near homogeneity Aquifex aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.01
-
 pH 7.9, 90°C, with Mg+ Aquifex aeolicus
22.8
-
 pH 7.9, 90°C, with Ca2+ Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-D-ribosylamine + Gly labile product Aquifex aeolicus ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Aquifex aeolicus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 enzyme is 8fold more active at 90°C than at 50°C Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 half life 60 h Aquifex aeolicus