Application | Comment | Organism |
---|---|---|
synthesis | efficient synthesis of gamma-glutamyl compounds by co-expression of gamma-glutamylmethylamide synthetase and polyphosphate kinase in engineered Escherichia coli | Methylovorus mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Methylovorus mays | |
Mg2+ | activates | Methylovorus mays | |
Mn2+ | activates | Methylovorus mays | |
additional information | no activation by Zn2+ and Ca2+ | Methylovorus mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
SDS-PAGE | Methylovorus mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylovorus mays | A9ZPH9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + ethylamine | - |
Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-Glutamylmethylamide synthetase | - |
Methylovorus mays |
GMAS | - |
Methylovorus mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Methylovorus mays |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | 35°C: about 75% of maximal activity, 55°C: about 60% of maximal activity | Methylovorus mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
3 h, less than 10% loss of activity | Methylovorus mays |
50 | - |
3 h, immediate loss of activity | Methylovorus mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Methylovorus mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 35% of maximal activity, pH 7.0, about 90% of maximal activity, pH 9.0: about 20% of maximal activity | Methylovorus mays |
6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: about 45% of maximal activity | Methylovorus mays |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
24 h, 40% loss of activity | Methylovorus mays |
9 | - |
24 h, complete inactivation | Methylovorus mays |