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Literature summary for 6.3.4.12 extracted from
- Theanine production by coupled fermentation with energy transfer using gamma-glutamylmethylamide synthetase of Methylovorus mays No. 9 (2008), Biosci. Biotechnol. Biochem., 72, 1206-1211.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the enzyme is usable in theanine formation by coupling with the alcoholic fermentation system of bakers yeast, Saccharomyces cerevisiae | Methylovorus mays |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain AD494(DE3) | Methylovorus mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | ethylamine inhibits yeast sugar fermentation and recombinant theanine production in vivo in a coupled theanine-foorming reaction with Saccharomyces cerevisiae, which can be overcome by potassium phosphate addition, overview | Methylovorus mays |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requred, slightly stimulating | Methylovorus mays |  |
| Mn2+ | stimulates | Methylovorus mays | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + ethylamine | Methylovorus mays | a step in theanine biosynthesis | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + ethylamine | Methylovorus mays No. 9 | a step in theanine biosynthesis | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + methylamine | Methylovorus mays | - |
ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
| ATP + L-glutamate + methylamine | Methylovorus mays No. 9 | - |
ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methylovorus mays | - |
- |
- |
| Methylovorus mays No. 9 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain AD494(DE3) by ammonium sulfate fractionation and anion exchange chromatography | Methylovorus mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamate + ethylamine | - |
Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + ethylamine | a step in theanine biosynthesis | Methylovorus mays | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + ethylamine | - |
Methylovorus mays No. 9 | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + ethylamine | a step in theanine biosynthesis | Methylovorus mays No. 9 | ADP + phosphate + N5-ethyl-L-glutamine | - |
? | |
| ATP + L-glutamate + methylamine | - |
Methylovorus mays | ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
| ATP + L-glutamate + methylamine | - |
Methylovorus mays No. 9 | ADP + phosphate + N5-methyl-L-glutamine | - |
? | |
| additional information | ammonia is a poor substrate | Methylovorus mays | ? | - |
? | |
| additional information | ammonia is a poor substrate | Methylovorus mays No. 9 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| gamma-Glutamylmethylamide synthetase | - |
Methylovorus mays |
| GMAS | - |
Methylovorus mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Methylovorus mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Methylovorus mays |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methylovorus mays | |
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