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Literature summary for 6.3.4.10 extracted from

  • Siegel, L.; Foote, J.L.; Coon, M.J.
    The enzymatic synthesis of propionyl coenzyme A holocarboxylase from d-biotinyl 5'-adenylate and the apocarboxylase (1965), J. Biol. Chem., 240, 1025-1031.
    View publication on PubMed
Search for more literature for 6.3.4.10

General Stability

General Stability Organism
loss of activity upon freezing Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuriphenylsulfonate
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000047
-
 biotin
-
 Oryctolagus cuniculus
0.000385
-
 ATP
-
 Oryctolagus cuniculus
0.0013
-
 ATP
-
 Oryctolagus cuniculus
0.0056
-
 biotinyl-5'-AMP
-
 Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Oryctolagus cuniculus
Mg2+ Mg2+ is probably required primarily for the synthesis of biotinyl-AMP from ATP and biotin, but not for the transfer of the biotin moiety from this intermediate to the apocarboxylase Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] Oryctolagus cuniculus
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus
apocarboxylase Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
0°C, 0.5 M sucrose, stable for about 1 week Oryctolagus cuniculus
4°C, stable for several weeks Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Rattus norvegicus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinyl-AMP is an intermediate Oryctolagus cuniculus AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 Oryctolagus cuniculus ?
-
 ?
biotinyl-5'-AMP + apocarboxylase
-
 Oryctolagus cuniculus AMP + holocarboxylase
-
 ?
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 19% of the activity relative to ATP Oryctolagus cuniculus CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 11% of the activity relative to ATP Oryctolagus cuniculus GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
UTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)] 24% of the activity relative to ATP Oryctolagus cuniculus UMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
53
-
 10-19% loss of activity after 1 min, 73-76% loss of activity after 5 min, 91-93% loss of activity after 15 min Oryctolagus cuniculus
53
-
 10% loss of activity after 1 min, 63% loss of activity after 5 min, 91% loss of activity after 15 min Oryctolagus cuniculus