BRENDA - Enzyme Database
show all sequences of 6.3.4.10

Purification and properties of bovine and human holocarboxylase synthetase

Suzuki, Y.; Narisawa, K.; Methods Enzymol. 279, 386-393 (1997)

Data extracted from this reference:

Application
Application
Commentary
Organism
diagnostics
the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
Bos taurus
diagnostics
the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
Homo sapiens
Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
General Stability
General Stability
Organism
after separation of the 64 kDa protein from an inert protein of 34 kDa on a nondenaturing PAGE, HCS is extremely labile and its enzymatic activity is totally lost within a few hours
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000013
-
biotin
; pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver
Bos taurus
0.000013
-
biotin
-
Oryctolagus cuniculus
20
-
ATP
pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration, HCS seems to be partially degraded, the molecular mass may be 81-85 kDa, the 64 kDa protein purified through gel filtration is clearly separated from an inert protein of 34 kDa on a nondenaturing PAGE at pH 9.5 or 8
Bos taurus
80759
-
1 * 80759, amino acid sequence calculation; x * 80759, calculation from nucleotide sequence
Homo sapiens
81000
85000
-
Homo sapiens
81000
85000
-
Oryctolagus cuniculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
-
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Homo sapiens
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Homo sapiens
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Rattus norvegicus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Rattus norvegicus
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Bos taurus
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
-
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC
1390
Homo sapiens
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC from biotin-deficient rat liver or human lymphoblast in the presence of d-biotin and ATP, much higher activity with apo-PCC from rat livers than from human lymphoblasts
1390
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ATP + biotin + apocarboxyl carrier protein
HCS catalyzes the incorporation of biotin into carboxylases, recombinant apo-CCP from Escherichia coli
1390
Bos taurus
AMP + diphosphate + holocarboxyl carrier protein
-
-
-
?
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
as active as ATP
1390
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
CTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
CTP can totally replace ATP
1390
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
50% of the activity relative to ATP
1390
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
GTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
GTP is approximately half as active as ATP
1390
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
50% of the activity relative to ATP
1390
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
ITP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
ITP is approximately half as active as ATP
1390
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 80759, calculation from nucleotide sequence
Homo sapiens
monomer
1 * 80759, amino acid sequence calculation
Homo sapiens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bos taurus
Application (protein specific)
Application
Commentary
Organism
diagnostics
the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
Bos taurus
diagnostics
the HCS assay using rat liver apopropionyl-CoA carboxylase as substrate is useful for enzymatic diagnosis of HCS deficiency
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
General Stability (protein specific)
General Stability
Organism
after separation of the 64 kDa protein from an inert protein of 34 kDa on a nondenaturing PAGE, HCS is extremely labile and its enzymatic activity is totally lost within a few hours
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.000013
-
biotin
; pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver
Bos taurus
0.000013
-
biotin
-
Oryctolagus cuniculus
20
-
ATP
pH 8, 30°C, cosubstrate apo-PCC from biotin-deficient rat liver
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
64000
-
gel filtration, HCS seems to be partially degraded, the molecular mass may be 81-85 kDa, the 64 kDa protein purified through gel filtration is clearly separated from an inert protein of 34 kDa on a nondenaturing PAGE at pH 9.5 or 8
Bos taurus
80759
-
1 * 80759, amino acid sequence calculation; x * 80759, calculation from nucleotide sequence
Homo sapiens
81000
85000
-
Homo sapiens
81000
85000
-
Oryctolagus cuniculus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
-
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Bos taurus
-
liver
-
Oryctolagus cuniculus
-
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
additional information
-
-
Oryctolagus cuniculus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Homo sapiens
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Homo sapiens
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Rattus norvegicus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Rattus norvegicus
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Bos taurus
-
ATP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
-
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC
1390
Homo sapiens
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ATP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
HCS catalyzes the incorporation of biotin into carboxylases, holo-PCC is formed from the apo-PCC from biotin-deficient rat liver or human lymphoblast in the presence of d-biotin and ATP, much higher activity with apo-PCC from rat livers than from human lymphoblasts
1390
Bos taurus
AMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ATP + biotin + apocarboxyl carrier protein
HCS catalyzes the incorporation of biotin into carboxylases, recombinant apo-CCP from Escherichia coli
1390
Bos taurus
AMP + diphosphate + holocarboxyl carrier protein
-
-
-
?
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
CTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
as active as ATP
1390
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
CTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
CTP can totally replace ATP
1390
Bos taurus
CMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
1390
Oryctolagus cuniculus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
GTP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
50% of the activity relative to ATP
1390
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
GTP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
GTP is approximately half as active as ATP
1390
Bos taurus
GMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
ITP + biotin + apo-[propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
50% of the activity relative to ATP
1390
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
-
ITP + biotin + apo-[propionyl-CoA:carbon-dioxide ligase (ADP-forming)]
ITP is approximately half as active as ATP
1390
Bos taurus
IMP + diphosphate + [propanoyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 80759, calculation from nucleotide sequence
Homo sapiens
monomer
1 * 80759, amino acid sequence calculation
Homo sapiens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Bos taurus
Other publictions for EC 6.3.4.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745784
Donti
Holocarboxylase synthetase de ...
Homo sapiens
Mol. Genet. Metab. Rep.
7
40-44
2016
-
1
-
-
4
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
744011
Miyao
Immobilization of immunoglobu ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii DSM 16993
Anal. Biochem.
484
113-121
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
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-
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726655
Xue
Holocarboxylase synthetase cat ...
Homo sapiens
Am. J. Physiol. Cell Physiol.
305
C1240-C1245
2013
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
728180
Li
Holocarboxylase synthetase int ...
Homo sapiens
J. Nutr. Biochem.
24
1446-1452
2013
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
-
-
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1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
713742
Singh
Identification of holocarboxyl ...
Homo sapiens
Anal. Biochem.
413
55-59
2011
-
1
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
714763
Rios-Avila
A 96-well plate assay for high ...
Homo sapiens
Clin. Chim. Acta
412
735-739
2011
-
1
1
-
-
-
-
1
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715997
Bao
Holocarboxylase synthetase is ...
Homo sapiens
J. Nutr. Biochem.
22
470-475
2011
-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
716237
Reyes-Carmona
Trafficking and chromatin dyna ...
Drosophila melanogaster
Mol. Genet. Metab.
103
240-248
2011
-
-
1
-
-
-
-
-
2
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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1
-
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2
-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
726859
Bao
Human holocarboxylase syntheta ...
Homo sapiens
Biochem. Biophys. Res. Commun.
412
115-120
2011
-
-
1
-
-
-
-
-
2
-
1
1
-
2
-
-
-
-
-
-
-
-
1
1
-
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-
-
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1
-
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-
1
1
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-
-
-
-
-
2
-
1
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701935
Hassan
The polypeptide Syn67 interact ...
Homo sapiens
Arch. Biochem. Biophys.
495
35-41
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
2
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