BRENDA - Enzyme Database show
show all sequences of 6.3.4.10

Biotin regulates the expression of holocarboxylase synthetase in the miR-539 pathway in HEK-293 cells

Bao, B.; Rodriguez-Melendez, R.; Wijeratne, S.; Zempleni, J.; J. Nutr. 140, 1546-1551 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
transgenic HEK-293 cells that overexpress HCS fused to green fluorescent protein
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
CACO-2 cell
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-
IMR-90 cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-
Cloned(Commentary) (protein specific)
Commentary
Organism
transgenic HEK-293 cells that overexpress HCS fused to green fluorescent protein
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
CACO-2 cell
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-
IMR-90 cell
-
Homo sapiens
-
JURKAT cell
-
Homo sapiens
-
Expression
Organism
Commentary
Expression
Homo sapiens
evolutionary conserved binding site for microRNA miR-539 in the 3'-untranslated region of HCS mRNA. miR-539 decreases the expression of HCS at the level of transcription rather than translation. When miR-539 is overexpressed in transgenic cells, the abundance of both HCS and biotinylated histones decreases. The abundance of miR-539 is tissue dependent, decreasing in the order fibroblasts, kidney cells, intestinal cells, lymphoid cells. The abundance of miR-539 is significantly higher at physiological concentrations of biotin than both biotin-deficient and biotin-supplemented media in all cell lines tested
down
Expression (protein specific)
Organism
Commentary
Expression
Homo sapiens
evolutionary conserved binding site for microRNA miR-539 in the 3'-untranslated region of HCS mRNA. miR-539 decreases the expression of HCS at the level of transcription rather than translation. When miR-539 is overexpressed in transgenic cells, the abundance of both HCS and biotinylated histones decreases. The abundance of miR-539 is tissue dependent, decreasing in the order fibroblasts, kidney cells, intestinal cells, lymphoid cells. The abundance of miR-539 is significantly higher at physiological concentrations of biotin than both biotin-deficient and biotin-supplemented media in all cell lines tested
down
Other publictions for EC 6.3.4.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745784
Donti
Holocarboxylase synthetase de ...
Homo sapiens
Mol. Genet. Metab. Rep.
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40-44
2016
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726655
Xue
Holocarboxylase synthetase cat ...
Homo sapiens
Am. J. Physiol. Cell Physiol.
305
C1240-C1245
2013
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728180
Li
Holocarboxylase synthetase int ...
Homo sapiens
J. Nutr. Biochem.
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Singh
Identification of holocarboxyl ...
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Rios-Avila
A 96-well plate assay for high ...
Homo sapiens
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Bao
Holocarboxylase synthetase is ...
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Reyes-Carmona
Trafficking and chromatin dyna ...
Drosophila melanogaster
Mol. Genet. Metab.
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Homo sapiens
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Hassan
The polypeptide Syn67 interact ...
Homo sapiens
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2010
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Holocarboxylase synthetase: co ...
Homo sapiens
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Lee
The N-terminal domain of human ...
Homo sapiens
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Wijeratne
K12-biotinylated histone H4 is ...
Homo sapiens
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715999
Bao
Biotin regulates the expressio ...
Homo sapiens
J. Nutr.
140
1546-1551
2010
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1
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702499
Zempleni
Biotin ...
Homo sapiens
Biofactors
35
36-46
2009
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702952
Yokoi
A case of holocarboxylase synt ...
Homo sapiens
Brain Dev.
31
775-778
2009
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703282
Tammachote
Holocarboxylase synthetase def ...
Homo sapiens
Clin. Genet.
78
88-93
2009
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Nyhan
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Hassan
N- and C-terminal domains in h ...
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2009
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Healy
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15
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Tissot
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1392
Dupuis
Clustering of mutations in the ...
Homo sapiens
Hum. Mol. Genet.
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1011-1016
1996
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