Protein Variants | Comment | Organism |
---|---|---|
H447A | the mutant shows severely reduced turnover number compared to the wild type enzyme | Streptomyces clavuligerus |
V446A | the mutant shows reduced turnover number compared to the wild type enzyme | Streptomyces clavuligerus |
V446G | the mutant shows strongly reduced turnover number compared to the wild type enzyme | Streptomyces clavuligerus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.04 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.04 | - |
(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.06 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.06 | - |
(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.08 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme H447A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.33 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.36 | - |
L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.46 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.6 | 1 | L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.9 | - |
L-N2-(2-carboxyethyl)arginine | mutant enzyme H447A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
1.2 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
1.9 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-N2-(2-carboxyethyl)arginine | Streptomyces clavuligerus | - |
AMP + diphosphate + deoxyamidinoproclavaminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | P0DJQ7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (2'R)-methyl-L-N2-(2-carboxyethyl)arginine | - |
Streptomyces clavuligerus | ? | - |
? | |
ATP + (2'S)-ethyl-L-N2-(2-carboxyethyl)arginine | - |
Streptomyces clavuligerus | ? | - |
? | |
ATP + (2'S)-methyl-L-N2-(2-carboxyethyl)arginine | - |
Streptomyces clavuligerus | ? | - |
? | |
ATP + L-N2-(2-carboxyethyl)arginine | - |
Streptomyces clavuligerus | AMP + diphosphate + deoxyamidinoproclavaminate | - |
? | |
additional information | no activity with (2'R)-ethyl-L-N2-(2-carboxyethyl)arginine | Streptomyces clavuligerus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-lactam-synthetase | - |
Streptomyces clavuligerus |
betaLS | - |
Streptomyces clavuligerus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.004 | - |
(2'S)-ethyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.013 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme H447A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.014 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.016 | - |
L-N2-(2-carboxyethyl)arginine | mutant enzyme H447A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.023 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.032 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.07 | - |
(2'R)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.13 | - |
L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.13 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446G, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.28 | - |
L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.34 | - |
(2'S)-methyl-L-N2-(2-carboxyethyl)arginine | mutant enzyme V446A, pH and temperature not specified in the publication | Streptomyces clavuligerus | |
0.87 | - |
L-N2-(2-carboxyethyl)arginine | wild type enzyme, pH and temperature not specified in the publication | Streptomyces clavuligerus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces clavuligerus |