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Literature summary for 6.3.3.3 extracted from

  • Eisenberg, M.A.; Krell, K.
    Dethiobiotin synthetase (1979), Methods Enzymol., 62, 348-352.
    View publication on PubMed
Search for more literature for 6.3.3.3

Inhibitors

Inhibitors Comment Organism Structure
ADP competitive to ATP Escherichia coli
dethiobiotin 10 mM, 30% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 7,8-diaminononanoate
-
 Escherichia coli
0.005
-
 ATP
-
 Escherichia coli
0.6
-
 Mg2+
-
 Escherichia coli
3.4
-
 CO2
-
 Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24500
-
 2 * 24500, SDS-PAGE Escherichia coli
42000
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 90% loss of activity after 24 h Escherichia coli
4°C, stable for about 2 weeks Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
ATP + diaminobiotin + CO2
-
 Escherichia coli ADP + phosphate + biotin
-
 ?
CTP + 7,8-diaminononanoate + CO2 50% of the activity relative to ATP Escherichia coli CDP + phosphate + dethiobiotin
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24500, SDS-PAGE Escherichia coli