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Literature summary for 6.3.3.3 extracted from

  • Izumi, Y.; Kano, Y.; Inagaki, K.; Kawase, N.; Tani, Y.; Yamada, H.
    Characterization of biotin biosynthetic enzymes of Bacillus sphaericus: a dethiobiotin producing bacterium (1981), Agric. Biol. Chem., 45, 1983-1989.
No PubMed abstract available
Search for more literature for 6.3.3.3

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 100% inhibition Lysinibacillus sphaericus
Cd2+ 1 mM, 70% inhibition Lysinibacillus sphaericus
Co2+ 1 mM, 35% inhibition Lysinibacillus sphaericus
Cu2+ 1 mM, 100% inhibition Lysinibacillus sphaericus
Hg2+ 1 mM, 100% inhibition Lysinibacillus sphaericus
Ni2+ 1 mM, 80% inhibition Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 7,8-diaminononanoate
-
 Lysinibacillus sphaericus
0.42
-
 ATP
-
 Lysinibacillus sphaericus
10
-
 CO2
-
 Lysinibacillus sphaericus
40
-
 MgCl2
-
 Lysinibacillus sphaericus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ required Lysinibacillus sphaericus
Mg2+ Km: 40 mM MgCl2 Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Alcaligenes faecalis
-
-
-
Bacillus roseus
-
-
-
Corynebacterium glutamicum
-
-
-
Escherichia coli
-
-
-
Klebsiella aerogenes
-
-
-
Klebsiella pneumoniae
-
-
-
Lipomyces starkeyi
-
-
-
Lysinibacillus sphaericus
-
-
-
Priestia megaterium
-
-
-
Pseudomonas taetrolens
-
-
-
Rhodotorula glutinis
-
-
-
Saccharomyces kloeckerianus
-
-
-
Sporidiobolus salmonicolor
-
-
-
Sporobolomyces coprophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli
additional information
-
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + 7,8-diaminononanoate + CO2 5% of the activity relative to ATP Lysinibacillus sphaericus AMP + phosphate + dethiobiotin
-
 ?
ADP + 7,8-diaminononanoate + CO2 5% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 AMP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Alcaligenes faecalis ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Escherichia coli ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Priestia megaterium ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Lysinibacillus sphaericus ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Klebsiella aerogenes ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Saccharomyces kloeckerianus ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Lipomyces starkeyi ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Sporidiobolus salmonicolor ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Sporobolomyces coprophilus ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Rhodotorula glutinis ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Klebsiella pneumoniae ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Bacillus roseus ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Corynebacterium glutamicum ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Pseudomonas taetrolens ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Lysinibacillus sphaericus ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2
-
 Lysinibacillus sphaericus IFO 3525 ADP + phosphate + dethiobiotin
-
 ?
ATP + 7,8-diaminononanoate + CO2 CO2 in form of HCO3- Lysinibacillus sphaericus IFO 3525 ADP + phosphate + dethiobiotin
-
 ?
ITP + 7,8-diaminononanoate + CO2 8.6% of the activity relative to ATP Lysinibacillus sphaericus IDP + phosphate + dethiobiotin
-
 ?
ITP + 7,8-diaminononanoate + CO2 8.6% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 IDP + phosphate + dethiobiotin
-
 ?
UTP + 7,8-diaminononanoate + CO2 7% of the activity relative to ATP Lysinibacillus sphaericus UDP + phosphate + dethiobiotin
-
 ?
UTP + 7,8-diaminononanoate + CO2 7% of the activity relative to ATP Lysinibacillus sphaericus IFO 3525 UDP + phosphate + dethiobiotin
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Lysinibacillus sphaericus