Literature summary for 6.3.3.3 extracted from

Muralla, R.; Chen, E.; Sweeney, C.; Gray, J.A.; Dickerman, A.; Nikolau, B.J.; Meinke, D.
A bifunctional locus (BIO3-BIO1) required for biotin biosynthesis in Arabidopsis (2008), Plant Physiol., 146, 60-73.

Cloned(Commentary)

Cloned (Comment)	Organism
gene bio3, alleles bio1-3, bio2-3, and bio3-3, DNA and amino acid sequence determination and analysis and genotyping, encoded with gene bio1 in a bifunctional locus that catalyzes two sequential reactions in the same metabolic pathway via differential splicing, separate BIO3 and BIO1 transcripts and two different types of chimeric BIO3-BIO1 transcripts are produced. One of the fused transcripts is monocistronic and encodes a bifunctional fusion protein. A splice variant is bicistronic, with distinct but overlapping reading frames, overview	Arabidopsis thaliana

Cloned (Comment)

Organism

gene bio3, alleles bio1-3, bio2-3, and bio3-3, DNA and amino acid sequence determination and analysis and genotyping, encoded with gene bio1 in a bifunctional locus that catalyzes two sequential reactions in the same metabolic pathway via differential splicing, separate BIO3 and BIO1 transcripts and two different types of chimeric BIO3-BIO1 transcripts are produced. One of the fused transcripts is monocistronic and encodes a bifunctional fusion protein. A splice variant is bicistronic, with distinct but overlapping reading frames, overview

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of bio3 insertion mutants, which have a similar phenotype to the bio1 and bio2 auxotrophs identified using forward genetic screens for arrested embryos rescued on enriched nutrient medium. Genes bio3 and bio1 mutants define a single genetic complementation group, separate BIO3 and BIO1 transcripts and two different types of chimeric BIO3-BIO1 transcripts are produced. One of the fused transcripts is monocistronic and encodes a bifunctional fusion protein. A splice variant is bicistronic, with distinct but overlapping reading frames. Dual functionality of the monocistronic transcript is confirmed by complementing the orthologous auxotrophs of Escherichia coli strain. Allelism between bio1 and bio3 heterozygotes and phenotypes, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	BIO3 protein contains an N-terminal sequence that is predicted to target the protein to mitochondria	Arabidopsis thaliana	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 7,8-diaminononanoate + CO2	Arabidopsis thaliana	third step in the biosynthesis of biotin, overview	ADP + phosphate + dethiobiotin	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	B0F481	BIO3; gene bio3	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
embryo	-	Arabidopsis thaliana	-
seedling	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 7,8-diaminononanoate + CO2	-	Arabidopsis thaliana	ADP + phosphate + dethiobiotin	-	?
ATP + 7,8-diaminononanoate + CO2	third step in the biosynthesis of biotin, overview	Arabidopsis thaliana	ADP + phosphate + dethiobiotin	-	?

Synonyms

Synonyms	Comment	Organism
BIO3	-	Arabidopsis thaliana
Dethiobiotin synthetase	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana