Literature summary for 6.3.3.3 extracted from

Alexeev, D.; Baxter, R.L.; Campopiano, D.J.; McAlpine, R.S.; McIver, L.; Sawyer, L.
Rational design of an inhibitor of dethiobiotin synthetase; interaction of 6-hydroxypyrimidin-4(3H)-one with the adenine base binding site (1998), Tetrahedron, 54, 15891-15898.

No PubMed abstract available

Search for more literature for 6.3.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are grown from ammonium sulfate, crystals are soaked in the crystallisation-well solution plus 50 mM 6-hydroxypyrimidin-4(3H)-one for 1 hour, 6-hydroxypyrimidin-4(3H)-one is embedded in the base binding pocket of DTBS	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
6-hydroxypyrimidin-4(3H)-one	competitive inhibition	Escherichia coli
adenine	-	Escherichia coli
AMP-PNP	-	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 7,8-diaminononanoate + CO2	Escherichia coli	penultimate step in biotin biosynthesis	ADP + phosphate + dethiobiotin	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant DTBS, ammonium sulfate, Sephacryl S-200, Q-Sepharose	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 7,8-diaminononanoate + CO2	-	Escherichia coli	ADP + phosphate + dethiobiotin	-	?
ATP + 7,8-diaminononanoate + CO2	penultimate step in biotin biosynthesis	Escherichia coli	ADP + phosphate + dethiobiotin	-	?

Synonyms

Synonyms	Comment	Organism
DTBS	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.05	-	AMP-PNP	25°C, pH 7.8	Escherichia coli
2.2	-	adenine	25°C, pH 7.8	Escherichia coli
11.2	-	6-hydroxypyrimidin-4(3H)-one	25°C, pH 7.8	Escherichia coli

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Release 2023.1 (January 2023)