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Literature summary for 6.3.3.3 extracted from
- Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis (1998), Protein Sci., 7, 2560-2566.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion, 0.005 ml of well solution containing 100 mM magnesium acetate or MgCl2, 9-11% polyethylene glycol 8000 and 100 mM cacodylate, pH 6.5 are mixed with 0.002 ml protein solution containing 30 mg/ml DTBS, crystals grow within a week at 20°C, crystals of DTBS complexed with diaminopelargonic acid-MgADP-AlF3 and with dethiobiotin-MgADP-phosphate, crystals diffract to 1.8 A | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7,8-diaminononanoate + CO2 | Escherichia coli | penultimate step in biotin biosynthesis | ADP + phosphate + dethiobiotin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7,8-diaminononanoate + CO2 | - |
Escherichia coli | ADP + phosphate + dethiobiotin | - |
? | |
| ATP + 7,8-diaminononanoate + CO2 | penultimate step in biotin biosynthesis | Escherichia coli | ADP + phosphate + dethiobiotin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DTBS | - |
Escherichia coli |
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