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Literature summary for 6.3.3.2 extracted from

  • Roje, S.; Janave, M.T.; Ziemak, M.J.; Hanson, A.D.
    Cloning and characterization of mitochondrial 5-formyltetrahydrofolate cycloligase from higher plants (2002), J. Biol. Chem., 277, 42748-42754.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis, expression in Escherichia coli BL21 as full length or truncated protein, the latter lacking the mitochondrial targeting sequence, functional complementation of enzyme deficient yeast fau1 mutant, yeast strain WHY1.3.1 Arabidopsis thaliana
DNA sequence determination and analysis, expression in Escherichia coli BL21(DE3) as full length or truncated protein, the latter lacking the mitochondrial targeting sequence Solanum lycopersicum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion exclusively Pisum sativum 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26500
-
1 * 26500, recombinant truncated enzyme, amino acid sequence determination Arabidopsis thaliana
27000
-
recombinant truncated enzyme, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-formyltetrahydrofolate Pisum sativum
-
ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate Arabidopsis thaliana
-
ADP + phosphate + 5,10-methenyltetrahydrofolate
-
ir
ATP + 5-formyltetrahydrofolate Solanum lycopersicum
-
ADP + phosphate + 5,10-methenyltetrahydrofolate
-
ir

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8L539
-
-
Pisum sativum
-
-
-
Solanum lycopersicum Q8LKF6
-
-

Purification (Commentary)

Purification (Comment) Organism
to near homogeneity, recombinant enzyme from Escherichia coli Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf high content Pisum sativum
-
root low content Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme Arabidopsis thaliana
additional information
-
activity of recombinant enzyme lacking the mitochondrial targeting sequence is significantly higher than the activity of the full length recombinant enzyme Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-formyltetrahydrofolate
-
Arabidopsis thaliana ADP + phosphate + 5,10-methylenetetrahydrofolate
-
ir
ATP + 5-formyltetrahydrofolate
-
Pisum sativum ADP + phosphate + 5,10-methenyltetrahydrofolate
-
?
ATP + 5-formyltetrahydrofolate
-
Arabidopsis thaliana ADP + phosphate + 5,10-methenyltetrahydrofolate
-
ir
ATP + 5-formyltetrahydrofolate
-
Solanum lycopersicum ADP + phosphate + 5,10-methenyltetrahydrofolate
-
ir
ATP + 5-formyltetrahydrofolate-pentaglutamate preferred substrate to the monoglutamyl form Arabidopsis thaliana ADP + phosphate + 5,10-methylenetetrahydrofolate-pentaglutamate
-
ir
additional information enzyme is specific for the 6S-isomer Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 26500, recombinant truncated enzyme, amino acid sequence determination Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
5-FCL
-
Pisum sativum
5-FCL
-
Arabidopsis thaliana
5-FCL
-
Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3
-
5-formyltetrahydrofolate truncated recombinant enzyme, pH 6.0, 23°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
bicine buffer Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 linear increase of activity to maximum at pH 8.5 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Pisum sativum
ATP dependent on Arabidopsis thaliana
ATP dependent on Solanum lycopersicum