Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus acidocaldarius |
expression in Escherichia coli | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
X-ray diffraction with resolution of 1.8 A of the enzyme complexed with LysW, L-glutamate, ADP, Mg2+, Zn2+ and sulfate. X-ray diffraction with resolution of 1.87 A of the enzyme complexed with ADP | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31534 | - |
4 * 31534, calculated from sequence | Sulfurisphaera tokodaii |
31715 | - |
x * 31715, calculated from sequence | Sulfolobus acidocaldarius |
126200 | - |
mass spectrometry | Sulfurisphaera tokodaii |
146000 | - |
analytical ultracentrifugation | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | Sulfurisphaera tokodaii | - |
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | Sulfolobus acidocaldarius | arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | Sulfolobus acidocaldarius DSM 639 | arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | Sulfurisphaera tokodaii 7 | - |
ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q4J8E7 | - |
- |
Sulfolobus acidocaldarius DSM 639 | Q4J8E7 | - |
- |
Sulfurisphaera tokodaii | Q970U6 | - |
- |
Sulfurisphaera tokodaii 7 | Q970U6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | - |
Sulfolobus acidocaldarius | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | - |
Sulfurisphaera tokodaii | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW | Sulfolobus acidocaldarius | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | - |
Sulfolobus acidocaldarius DSM 639 | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW | Sulfolobus acidocaldarius DSM 639 | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? | |
ATP + [LysW]-C-terminal-L-glutamate + L-glutamate | - |
Sulfurisphaera tokodaii 7 | ADP + phosphate + [LysW]-C-terminal-gamma-(L-glutamyl)-L-glutamate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31715, calculated from sequence | Sulfolobus acidocaldarius |
tetramer | 4 * 31534, calculated from sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ArgX | - |
Sulfolobus acidocaldarius |
Saci_1621 | locus name | Sulfolobus acidocaldarius |
StArgX | - |
Sulfurisphaera tokodaii |
STK_15050 | - |
Sulfurisphaera tokodaii |
General Information | Comment | Organism |
---|---|---|
malfunction | no growth of deletion mutant without L-arginine. Growth of the deletion mutant with L-arginine supplementation is possible | Sulfolobus acidocaldarius |
physiological function | arginine biosynthesis. Protection of the amino group of L-glutamate by conjugation to the carrier protein LysW | Sulfolobus acidocaldarius |