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Literature summary for 6.3.2.9 extracted from
- Over-production, purification and properties of the uridine diphosphate N-acetylmuramoyl-L-alanine:D-glutamate ligase from Escherichia coli (1991), Eur. J. Biochem., 202, 1169-1176.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| potassium phosphate | highly dependent on, optimal concentration: 11-16 mM, inhibition above 20 mM, phosphate ion is responsible for inhibition | Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0075 | - |
UDP-N-acetylmuramoyl-L-Ala | - |
Escherichia coli | |
| 0.055 | - |
D-Glu | - |
Escherichia coli | |
| 0.138 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | highly dependent on, optimal concentration: 5 mM | Escherichia coli | |
| potassium phosphate | highly dependent on, optimal concentration: 11-16 mM, inhibition above 20 mM | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 Km83, an overproducing strain that harbours recombinant plasmids bearing the murD gene under the control of the lac Pg promoter, JM83(pDML13) and JM83(pMLD50) | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.261 | - |
Escherichia coli JM83(pDML13) | Escherichia coli |
| 1.92 | - |
Escherichia coli JM83 (pMLD50) | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate | ATP in form of MgATP2- | Escherichia coli | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate | - |
? | |
| ATP + UDP-N-acetylmuramoyl-L-Ala + Glu | - |
Escherichia coli | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | 9.2 | - |
Escherichia coli |
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