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Literature summary for 6.3.2.8 extracted from

  • Videau, P.; Rivers, O.S.; Ushijima, B.; Oshiro, R.T.; Kim, M.J.; Philmus, B.; Cozy, L.M.
    Mutation of the murC and murB genes impairs heterocyst differentiation in Anabaena sp. strain PCC 7120 (2016), J. Bacteriol., 198, 1196-1206 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview Nostoc sp. PCC 7120 = FACHB-418

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Nostoc sp. PCC 7120 = FACHB-418

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine Nostoc sp. PCC 7120 = FACHB-418
-
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576
-
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
?

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q8YM75
-
-
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 Q8YM75
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
-
Nostoc sp. PCC 7120 = FACHB-418 ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
?
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine
-
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
-
?

Synonyms

Synonyms Comment Organism
alr5065
-
Nostoc sp. PCC 7120 = FACHB-418
MurC
-
Nostoc sp. PCC 7120 = FACHB-418
UDP-N-acetylmuramate:L-alanine ligase
-
Nostoc sp. PCC 7120 = FACHB-418

Cofactor

Cofactor Comment Organism Structure
ATP
-
Nostoc sp. PCC 7120 = FACHB-418

General Information

General Information Comment Organism
malfunction mutation of the gene murC impairs heterocyst differentiation in Nostoc sp. PCC 7120. Gene murC depletion leads to aberrant expression of patS in all cells of the filament Nostoc sp. PCC 7120 = FACHB-418
metabolism enzyme MurC catalyzes the third step in peptidoglycan biosynthesis Nostoc sp. PCC 7120 = FACHB-418
physiological function the enzyme is important in peptidoglycan biosynthesis. Heterocyst development has been shown to require proper peptidoglycan remodeling. Determination of a direct link between peptidoglycan synthesis and the maintenance of a biological pattern in a multicellular organism. Nostoc sp. PCC 7120 differentiates a periodic pattern of specialized heterocyst cells, requiring peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development, overview Nostoc sp. PCC 7120 = FACHB-418