Protein Variants | Comment | Organism |
---|---|---|
additional information | screening and identification of a transposon mutant impaired in diazotrophic growth, the mutant strain contains a transposon insertion at nucleotide position -476 relative to the annotated translational start site of gene alr5065. The mutant, designated C6, is unable to grow diazotrophically and displays a deficit in heterocyst differentiation, producing 4.5% heterocysts compared to the 9% produced by the wild type. Proper heterocyst production is restored in mutant C6 when the mutation is complemented with both alr5065 and alr5066, encoding enzymes MurC and MurB, as PpetE-murC/B (pPJAV328) in the presence of copper, but not when either gene is introduced individually, which suggests that alr5065 and alr5066 may be in an operon and the transposon insertion affectes the expression of both genes. After repeated rounds of subculturing, C6 loses viability and ceases to grow in culture. Generation of murC mutant strain UHM351, in which murC is inactivated by pPJAV309, phenotype, overview | Nostoc sp. PCC 7120 = FACHB-418 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Nostoc sp. PCC 7120 = FACHB-418 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Nostoc sp. PCC 7120 = FACHB-418 | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | - |
ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc sp. PCC 7120 = FACHB-418 | Q8YM75 | - |
- |
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | Q8YM75 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Nostoc sp. PCC 7120 = FACHB-418 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine | - |
Nostoc sp. PCC 7120 = FACHB-418 SAG 25.82 / UTEX 2576 | ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alr5065 | - |
Nostoc sp. PCC 7120 = FACHB-418 |
MurC | - |
Nostoc sp. PCC 7120 = FACHB-418 |
UDP-N-acetylmuramate:L-alanine ligase | - |
Nostoc sp. PCC 7120 = FACHB-418 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Nostoc sp. PCC 7120 = FACHB-418 |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation of the gene murC impairs heterocyst differentiation in Nostoc sp. PCC 7120. Gene murC depletion leads to aberrant expression of patS in all cells of the filament | Nostoc sp. PCC 7120 = FACHB-418 |
metabolism | enzyme MurC catalyzes the third step in peptidoglycan biosynthesis | Nostoc sp. PCC 7120 = FACHB-418 |
physiological function | the enzyme is important in peptidoglycan biosynthesis. Heterocyst development has been shown to require proper peptidoglycan remodeling. Determination of a direct link between peptidoglycan synthesis and the maintenance of a biological pattern in a multicellular organism. Nostoc sp. PCC 7120 differentiates a periodic pattern of specialized heterocyst cells, requiring peptidoglycan synthesis by the murine ligase genes murC (alr5065) and murB (alr5066) for maintenance of patterned gene expression, filament integrity, and overall development, overview | Nostoc sp. PCC 7120 = FACHB-418 |