Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
UDP-N-acetylmuramate | pH 8.6, 37°C | Staphylococcus aureus | |
0.44 | - |
L-Ala | pH 8.6, 37°C | Staphylococcus aureus | |
2 | - |
ATP | pH 8.6, 37°C | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, optimal concentration 5 mM | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + 2-amino-N-butyric acid | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-2-amino-N-butyric acid | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | accepts L-Ala, L-Ser and Gly as substrates, with a strong preference for L-Ala | Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.8 | - |
Staphylococcus aureus |