Cloned (Comment) | Organism |
---|---|
gene murC, expression of N-terminally His-tagged wild-type and mutant enzymes as soluble proteins in Escherichia coli strain BL21(DE3), cloning in Corynebacterium glutamicum strain ATCC 13869 | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of the phosphorylation site threonine residues highly impairs enzyme activity | Corynebacterium glutamicum |
T362A/T365A/T51A | site-directed mutagenesis, the mutant shows impaired activity | Corynebacterium glutamicum |
T362A/T365A/T51A/T120A | site-directed mutagenesis, the mutant shows impaired activity | Corynebacterium glutamicum |
T362A/T365A/T51A/T120A/T167A | site-directed mutagenesis, the mutant shows impaired activity | Corynebacterium glutamicum |
T362A/T365A/T51A/T120A/T167A/T133A | site-directed mutagenesis, the mutant shows impaired activity | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, recombinant detagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + L-alanine | Corynebacterium glutamicum | MurC is essential for peptidoglycan biosynthesis and is responsible of the addition of the first residue L-alanine onto the nucleotide precursor UDP-MurNAc. It is regulated by the serine/threonine protein kinase PknA, overview | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine | - |
? | |
additional information | Corynebacterium glutamicum | regulation by reversible phosphorylation, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene murC | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | MurC is phosphorylated in vitro by the PknA protein kinase, but not by PknG kinase, exclusively on threonine residues, which has regulatory function, mutation of the phosphorylation site threonine residues impairs enzyme activity, double phosphorylation of the activation loop residues Thr179 and Thr181 is necessary for full kinase activity | Corynebacterium glutamicum |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), the His-tag is cleaved off by TEV protease | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + L-alanine | - |
Corynebacterium glutamicum | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine | - |
? | |
ATP + UDP-N-acetylmuramate + L-alanine | MurC is essential for peptidoglycan biosynthesis and is responsible of the addition of the first residue L-alanine onto the nucleotide precursor UDP-MurNAc. It is regulated by the serine/threonine protein kinase PknA, overview | Corynebacterium glutamicum | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine | - |
? | |
additional information | regulation by reversible phosphorylation, overview | Corynebacterium glutamicum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant detagged enzyme, SDS-PAGE | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
MurC ligase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Corynebacterium glutamicum |