Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.2.8 extracted from

  • Hishinuma, F.; Izaki, K.; Takahashi, H.
    Inhibition of L-alanine adding enzyme by glycine (1971), Agric. Biol. Chem., 35, 2050-2058.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Bacillus subtilis
Cys stimulates Bacillus subtilis
dithiothreitol stimulates Bacillus subtilis
glutathione stimulates Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Bacillus subtilis
Cu2+
-
Bacillus subtilis
Gly
-
Bacillus cereus
Gly
-
Bacillus subtilis
L-Ser inhibits reaction with Gly + N-acetylmuramate + ATP Bacillus subtilis
NEM
-
Bacillus subtilis
Ni2+
-
Bacillus subtilis
PCMB
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-Ala
-
Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight stimulation Bacillus subtilis
Mg2+ optimal concentration: 10 mM Bacillus subtilis
Mg2+ divalent cation required, Mg2+ or Mn2+ Bacillus subtilis
Mn2+ divalent cation required Bacillus subtilis
Mn2+ optimal concentration: 2-5 mM Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Bacillus subtilis
partial Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.413
-
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + UDP-N-acetylmuramate + Gly
-
Bacillus subtilis ADP + phosphate + UDP-N-acetylmuramoyl-Gly
-
?
ATP + UDP-N-acetylmuramate + L-Ala
-
Bacillus subtilis ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
?
ATP + UDP-N-acetylmuramate + L-Ala
-
Bacillus cereus ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
Bacillus subtilis