Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates | Bacillus subtilis | |
Cys | stimulates | Bacillus subtilis | |
dithiothreitol | stimulates | Bacillus subtilis | |
glutathione | stimulates | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bacillus subtilis | |
Cu2+ | - |
Bacillus subtilis | |
Gly | - |
Bacillus cereus | |
Gly | - |
Bacillus subtilis | |
L-Ser | inhibits reaction with Gly + N-acetylmuramate + ATP | Bacillus subtilis | |
NEM | - |
Bacillus subtilis | |
Ni2+ | - |
Bacillus subtilis | |
PCMB | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-Ala | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight stimulation | Bacillus subtilis | |
Mg2+ | optimal concentration: 10 mM | Bacillus subtilis | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Bacillus subtilis | |
Mn2+ | divalent cation required | Bacillus subtilis | |
Mn2+ | optimal concentration: 2-5 mM | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Bacillus subtilis |
partial | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.413 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + Gly | - |
Bacillus subtilis | ADP + phosphate + UDP-N-acetylmuramoyl-Gly | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | - |
Bacillus subtilis | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | - |
Bacillus cereus | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Bacillus subtilis |