Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates | Staphylococcus aureus | |
2-mercaptoethanol | maximal concentration 2.5 mM | Staphylococcus aureus |
General Stability | Organism |
---|---|
decrease in stability at high salt concentrations is prevented by addition of 10% glycerol and 2.5 mM 2-mercaptoethanol | Staphylococcus aureus |
relatively stable at low ionic strength, unstable at high concentrations of salt | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Ca2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Cd2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Co2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Fe2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Hg2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Mg2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Ni2+ | inhibits activation by Mn2+ | Staphylococcus aureus | |
Zn2+ | inhibits activation by Mn2+ | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.051 | - |
UDP-N-acetylmuramate | - |
Staphylococcus aureus | |
0.15 | - |
L-Ala | - |
Staphylococcus aureus | |
29 | - |
Gly | - |
Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | monovalent cation required, NH4+ or K+ | Staphylococcus aureus | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Staphylococcus aureus | |
Mn2+ | divalent cation required | Staphylococcus aureus | |
Mn2+ | Mg2+ or Mn2+, maximal activity at concentrations of Mn2+ equimolar to those of ATP | Staphylococcus aureus | |
NH4+ | monovalent cation required, NH4+ or K+ | Staphylococcus aureus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration in absence and in presence of 2.5 mM 2-mercaptoethanol | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
- |
Staphylococcus aureus |
Storage Stability | Organism |
---|---|
-18°C, 5 mM potassium phosphate, 10-20% loss of activity after 3 months | Staphylococcus aureus |
4°C, 5 mM potassium phosphate, overnight, 10-20% loss of activity. At 4°C, 250 mM potassium phosphate, 12 h, 30% loss of activity | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UDP-N-acetylmuramate + DL-Ser | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-DL-Ser | - |
? | |
ATP + UDP-N-acetylmuramate + Gly | - |
Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-Gly | - |
? | |
ATP + UDP-N-acetylmuramate + L-Ala | not active with D-Ala | Staphylococcus aureus | ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? | |
UTP + UDP-N-acetylmuramate + L-Ala | about 11% of the activity relative to ATP | Staphylococcus aureus | UDP + phosphate + UDP-N-acetylmuramoyl-L-Ala | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 mM potassium phosphate, pH 7.2, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | pH 7.5: about 60% of maximal activity, pH 9.0: about 70% of maximal activity | Staphylococcus aureus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
5 mM potassium phosphate, 50°C, 20 min, 10-20% loss of activity. 60-90% loss of activity in 250 mM potassium phosphate buffer | Staphylococcus aureus |