Literature summary for 6.3.2.7 extracted from

Blewett, A.M.; Lloyd, A.J.; Echalier, A.; Fulop, V.; Dowson, C.G.; Bugg, T.D.; Roper, D.I.
Expression, purification, crystallization and preliminary characterization of uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase (MurE) from Streptococcus pneumoniae 110K/70 (2004), Acta Crystallogr. Sect. D, 60, 359-361.

Cloned(Commentary)

Cloned (Comment)	Organism
cloned into the high-expression plasmid pET21b and overexpressed in Escherichia coli BL21 (DE3) Star	Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are obtained by the hanging-drop vapour-diffusion method at 291 K from solutions containing 25%(w/v) polyethylene glycol 2000 monomethylether, 0.2 M potassium thiocyanate, 0.1 M MES pH 6.5 in the presence of uridine 5'-diphospho-N-acetylmuramoyl alanyl glutamate (UDP-MurNAc-l-Ala-d-Glu) with and without 5'-adenylyl imidophosphate (AMP-PNP), a non-hydrolysable analogue of ATP. Crystals grown in the presence of two ligands belong to space group P1, with unit-cell parameters a = 68.4 A, b = 71.4 A, c = 74.8 A, alpha = 73.4, beta = 80.5, gamma = 72.3 A. Crystals grown in the presence of UDP-MurNAc-l-Ala-d-Glu alone belong to space group P2(1), with unit-cell parameters a = 71.1 A, b = 129.4 A, c = 74.6 A, beta = 106.3°	Streptococcus pneumoniae

Crystallization (Comment)

Organism

crystals are obtained by the hanging-drop vapour-diffusion method at 291 K from solutions containing 25%(w/v) polyethylene glycol 2000 monomethylether, 0.2 M potassium thiocyanate, 0.1 M MES pH 6.5 in the presence of uridine 5'-diphospho-N-acetylmuramoyl alanyl glutamate (UDP-MurNAc-l-Ala-d-Glu) with and without 5'-adenylyl imidophosphate (AMP-PNP), a non-hydrolysable analogue of ATP. Crystals grown in the presence of two ligands belong to space group P1, with unit-cell parameters a = 68.4 A, b = 71.4 A, c = 74.8 A, alpha = 73.4, beta = 80.5, gamma = 72.3 A. Crystals grown in the presence of UDP-MurNAc-l-Ala-d-Glu alone belong to space group P2(1), with unit-cell parameters a = 71.1 A, b = 129.4 A, c = 74.6 A, beta = 106.3°

Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
MuE	-	Streptococcus pneumoniae
uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)