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Literature summary for 6.3.2.61 extracted from

  • Regnard, C.; Desbruyeres, E.; Denoulet, P.; Edde, B.
    Tubulin polyglutamylase isozymic variants and regulation during the cell cycle in HeLa cells (1999), J. Cell Sci., 112, 4281-4289 .
    View publication on PubMed
Search for more literature for 6.3.2.61

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 8 mM used in assay conditions Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
n ATP + [alpha-tubulin]-L-glutamate + n L-glutamate Mus musculus overall reaction [alpha-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate Mus musculus overall reaction [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by phosphocellulose column chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is able to glutamylate both alpha- and beta-tubulin from mouse brain but only alpha-tubulin from HeLa cells Mus musculus ?
-
 ?
n ATP + [alpha-tubulin]-L-glutamate + n L-glutamate overall reaction Mus musculus [alpha-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?
n ATP + [beta-tubulin]-L-glutamate + n L-glutamate overall reaction Mus musculus [beta-tubulin]-(gamma-(poly-alpha-L-glutamyl)-L-glutamyl)-L-glutamate + n ADP + n phosphate
-
 ?

Synonyms

Synonyms Comment Organism
alpha-TPG
-
 Mus musculus
tubulin polyglutamylase
-
 Mus musculus

Expression

Organism Comment Expression
Mus musculus enzyme activity is maximal in G2-phase and decreases during M-phase of the cell cycle up