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Literature summary for 6.3.2.6 extracted from

  • Tuntland, M.L.; Fung, L.W.
    Substrate independent ATPase activity may complicate high throughput screening (2016), Anal. Biochem., 510, 18-20 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.6

Inhibitors

Inhibitors Comment Organism Structure
additional information malachite green assay (MGA) in a high throughput screening (HTS) setup to identify compounds that inhibit the activity of Bacillus anthracis PurC (BaPurC) Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Bacillus anthracis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Bacillus anthracis
-
 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81ZH5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
 Bacillus anthracis ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
 ?
ATP + H2O
-
 Bacillus anthracis ADP + phosphate
-
 ?
additional information usage of the malachite green assay screening method for detection of inhibitors of SAICAR synthetase (PurC). A gradual increase in inaorganic phosphate occurs in positive control samples over the course of the day. Further investigation indicates that hydrolysis of ATP catalyzed by PurC, rather than substrate-related phosphate release, is responsible for a partial contribution to the signals in the control samples. Thus substrate-independent ATPase activity may complicate high throughput screening Bacillus anthracis ?
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-

Synonyms

Synonyms Comment Organism
ATPase
-
 Bacillus anthracis
BaPurC
-
 Bacillus anthracis
PurC
-
 Bacillus anthracis
SAICAR synthetase
-
 Bacillus anthracis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bacillus anthracis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus anthracis

General Information

General Information Comment Organism
metabolism SAICAR synthetase (PurC) is involved in de novo purine biosynthesis Bacillus anthracis
physiological function the enzyme catalyzes direct activation of the carboxylate of CAIR by the gamma-phosphate of ATP, leading to the formation of products, SAICAR, ADP and phosphate Bacillus anthracis