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Literature summary for 6.3.2.6 extracted from

  • Wolf, N.M.; Abad-Zapatero, C.; Johnson, M.E.; Fung, L.W.
    Structures of SAICAR synthetase (PurC) from Streptococcus pneumoniae with ADP, Mg2+, AIR and Asp (2014), Acta Crystallogr. Sect. D, 70, 841-850 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene purC, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21-Gold Streptococcus pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His-tagged enzyme complexed with ADP or ADP, L-Asp and 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, hanging drop vapour diffusion method, mixing of 0.002 ml of 0.25 mM protein in 15 mM Tris, pH 8.0, 25 mM KCl, 55 mM MgCl2, 5 mM EDTA, and 5 mM DTT, with 0.002 ml of reservoir solution containing 0.16 M sodium acetate, pH 4.5, 36-40% w/v PEG 200, plus mM ADP, 1 mM CAIR, and 4 mM Asp or plus 10 mM ADP, X-ray diffraction structure determination and analysis at 2.3-2.69 A resolution, modeling, molecular replacement using the Escherichia coli enzyme structure, PDB ID 2gqr, as search model Streptococcus pneumoniae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae
-
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate Streptococcus pneumoniae ATCC 33400
-
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
-
-
Streptococcus pneumoniae ATCC 33400
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold by nickel affinity chromatography and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
Streptococcus pneumoniae ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate
-
Streptococcus pneumoniae ATCC 33400 ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate
-
?

Synonyms

Synonyms Comment Organism
PurC
-
Streptococcus pneumoniae
SAICAR synthetase
-
Streptococcus pneumoniae
spPurC
-
Streptococcus pneumoniae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Streptococcus pneumoniae

General Information

General Information Comment Organism
metabolism the seventh step of the de novo purine-biosynthesis pathway converts carboxyaminoimidazoleribonucleotide (CAIR) and L-aspartic acid (Asp) to 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide (SAICAR) in the presence of adenosine 5'-triphosphate (ATP) using the enzyme PurC Streptococcus pneumoniae
additional information modeling of two structures for the active site with all of the essential ligands (ATP, Mg2+, Asp and CAIR) and of a relay mechanism for the formation of the product (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate (SAICAR), active site structure analysis, overview Streptococcus pneumoniae
physiological function enzyme PurC is conditionally essential for bacterial replication Streptococcus pneumoniae