Cloned (Comment) | Organism |
---|---|
gene purC, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21-Gold | Streptococcus pneumoniae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme complexed with ADP or ADP, L-Asp and 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, hanging drop vapour diffusion method, mixing of 0.002 ml of 0.25 mM protein in 15 mM Tris, pH 8.0, 25 mM KCl, 55 mM MgCl2, 5 mM EDTA, and 5 mM DTT, with 0.002 ml of reservoir solution containing 0.16 M sodium acetate, pH 4.5, 36-40% w/v PEG 200, plus mM ADP, 1 mM CAIR, and 4 mM Asp or plus 10 mM ADP, X-ray diffraction structure determination and analysis at 2.3-2.69 A resolution, modeling, molecular replacement using the Escherichia coli enzyme structure, PDB ID 2gqr, as search model | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Streptococcus pneumoniae | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Streptococcus pneumoniae ATCC 33400 | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Streptococcus pneumoniae ATCC 33400 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold by nickel affinity chromatography and gel filtration | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Streptococcus pneumoniae | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? | |
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Streptococcus pneumoniae ATCC 33400 | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PurC | - |
Streptococcus pneumoniae |
SAICAR synthetase | - |
Streptococcus pneumoniae |
spPurC | - |
Streptococcus pneumoniae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
metabolism | the seventh step of the de novo purine-biosynthesis pathway converts carboxyaminoimidazoleribonucleotide (CAIR) and L-aspartic acid (Asp) to 4-(N-succino)-5-aminoimidazole-4-carboxamide ribonucleotide (SAICAR) in the presence of adenosine 5'-triphosphate (ATP) using the enzyme PurC | Streptococcus pneumoniae |
additional information | modeling of two structures for the active site with all of the essential ligands (ATP, Mg2+, Asp and CAIR) and of a relay mechanism for the formation of the product (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate (SAICAR), active site structure analysis, overview | Streptococcus pneumoniae |
physiological function | enzyme PurC is conditionally essential for bacterial replication | Streptococcus pneumoniae |