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Literature summary for 6.3.2.6 extracted from

  • Humble, R.W.; Mackenzie, G.; Shaw, G.
    Rapid purification of a bifunctional protein complex possessing phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (1991), Adv. Exp. Med. Biol., 309B, 223-227.
    View publication on PubMed
Search for more literature for 6.3.2.6

General Stability

General Stability Organism
DTT is essential in maintaining the stability, in absence of DTT the activity is completely lost within two hours of extraction Ovis aries
the ionic strength required to maintain enzyme activity is 0.1 M Tris HCl, at pH 7.8 Ovis aries

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Ovis aries

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 gradient gel electrophoresis of the native protein Ovis aries
420000
-
 gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
copurification of the bifunctional protein complex possessing phosphoribosylaminoimidazole carboxylase (EC 4.1.1.21) and phosphoribosylaminoimidazolesuccinocarboxamide synthetase (EC 6.3.2.6) Ovis aries

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.06
-
-
 Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-(5'-phosphoribosyl)-5-amino-4-carboxyimidazole + L-Asp
-
 Ovis aries ADP + phosphate + 1-(5'-phosphoribosyl)-5-amino-4-(N-succinocarboxamide)-imidazole
-
 ?

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.4 8 narrow stability profile with maximal stability at pH 7.6 Ovis aries