Protein Variants | Comment | Organism |
---|---|---|
additional information | the Pyl operon in Methanosarcina species contains five genes encoding three biosynthetic enzymes (PylB, PylC and PylD), a specific pyrrolysyl-tRNA synthetase (PylS) and the tRNA (PylT). Transfer of these genes is necessary and sufficient to support incorporation of Pyl intoproteins in Escherichia coli at low yields. Addition of D-ornithine to culture medium has been shown to increase yields of full-length protein expression in Escherichia coli containing the Pyl synthesis genes. Development of plasmid systems with inducible promoters for high-level protein expression in both Escherichia coli and mammalian cells (HEK-293 cells) using genes from Methanosarcina mazei, overview. A new amino acid, S-2-amino-6-(R-3,4-dihydro-2H-pyrrole-2-carboxamido)-hexanoic acid, named pyrroline-carboxy-lysine (Pcl), is only produced in high amounts in the presence of D-ornithine but, like Pyl, is a substrate for PylS for site-specific incorporation into proteins | Methanosarcina mazei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Methanosarcina mazei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (3R)-3-methyl-D-ornithine + L-lysine | Methanosarcina mazei | - |
ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
additional information | Methanosarcina mazei | the amino acid, S-2-amino-6-(R-3,4-dihydro-2H-pyrrole-2-carboxamido)-hexanoic acid, named pyrroline-carboxy-lysine (Pcl), is only produced in high amounts in the presence of D-ornithine but, like Pyl, is a substrate for PylS for site-specific incorporation into proteins | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina mazei | A0A4P8QYJ1 | Methanosarcina frisia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (3R)-3-methyl-D-ornithine + L-lysine | - |
Methanosarcina mazei | ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine | - |
? | |
additional information | the amino acid, S-2-amino-6-(R-3,4-dihydro-2H-pyrrole-2-carboxamido)-hexanoic acid, named pyrroline-carboxy-lysine (Pcl), is only produced in high amounts in the presence of D-ornithine but, like Pyl, is a substrate for PylS for site-specific incorporation into proteins | Methanosarcina mazei | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
3-methylornithine-L-lysine ligase | UniProt | Methanosarcina mazei |
pylC | - |
Methanosarcina mazei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanosarcina mazei |
General Information | Comment | Organism |
---|---|---|
metabolism | proposed pathway for the biosynthesis of amino acids Pyl and S-2-amino-6-(R-3,4-dihydro-2H-pyrrole-2-carboxamido)-hexanoic acid, named pyrroline-carboxy-lysine (Pcl), overview. PylB does not act on Pcl or L-Lys-D-Orn but rather acts upstream of PylC and PylD. For this to occur, PylC and PylD must tolerate methylated intermediates. D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine | Methanosarcina mazei |
additional information | analysis of the incorporation of Pcl and Pyl at TAG codons, tandem mass spectrometry | Methanosarcina mazei |