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Literature summary for 6.3.2.59 extracted from

  • Gaston, M.; Jiang, R.; Krzycki, J.
    Functional context, biosynthesis, and genetic encoding of pyrrolysine (2011), Curr. Opin. Microbiol., 14, 342-349 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.59

Protein Variants

Protein Variants Comment Organism
additional information Escherichia coli expressing archaeal pylTSBCD can incorporate biosynthesized pyrrolysine into protein, with pylBCD required to make the PylS substrate Methanococcoides burtonii
additional information Escherichia coli expressing archaeal pylTSBCD can incorporate biosynthesized pyrrolysine into protein, with pylBCD required to make the PylS substrate Methanohalophilus mahii
additional information Escherichia coli expressing archaeal pylTSBCD can incorporate biosynthesized pyrrolysine into protein, with pylBCD required to make the PylS substrate Methanohalobium evestigatum
additional information Escherichia coli expressing archaeal pylTSBCD can incorporate biosynthesized pyrrolysine into protein, with pylBCD required to make the PylS substrate Methanosarcina spp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Methanococcoides burtonii
Mg2+ required Methanohalophilus mahii
Mg2+ required Methanohalobium evestigatum
Mg2+ required Methanosarcina spp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanococcoides burtonii
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanohalophilus mahii
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanohalobium evestigatum
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanosarcina spp.
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanococcoides burtonii OCM 468
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanococcoides burtonii ACE-M
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanococcoides burtonii NBRC 107633
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanohalobium evestigatum DSM 3721
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanohalophilus mahii ATCC 35705
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine Methanococcoides burtonii DSM 6242
-
 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Methanococcoides burtonii Q12UB8
-
-
Methanococcoides burtonii ACE-M Q12UB8
-
-
Methanococcoides burtonii DSM 6242 Q12UB8
-
-
Methanococcoides burtonii NBRC 107633 Q12UB8
-
-
Methanococcoides burtonii OCM 468 Q12UB8
-
-
Methanohalobium evestigatum D7E781
-
-
Methanohalobium evestigatum DSM 3721 D7E781
-
-
Methanohalophilus mahii D5E9G2
-
-
Methanohalophilus mahii ATCC 35705 D5E9G2
-
-
Methanosarcina spp.
-
-
-
no activity in Desulfobacterium autotrophicum
-
 contains no gene pylC
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanococcoides burtonii ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanohalophilus mahii ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanohalobium evestigatum ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanosarcina spp. ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanococcoides burtonii OCM 468 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanococcoides burtonii ACE-M ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanococcoides burtonii NBRC 107633 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanohalobium evestigatum DSM 3721 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanohalophilus mahii ATCC 35705 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?
ATP + (3R)-3-methyl-D-ornithine + L-lysine
-
 Methanococcoides burtonii DSM 6242 ADP + phosphate + N6-[(3R)-3-methyl-D-ornithinyl]-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
ATP-grasp domain-containing protein UniProt Methanohalophilus mahii
ATP-grasp domain-containing protein UniProt Methanohalobium evestigatum
pylC
-
 Methanococcoides burtonii
pylC
-
 Methanohalophilus mahii
pylC
-
 Methanohalobium evestigatum
pylC
-
 Methanosarcina spp.

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanococcoides burtonii
ATP
-
 Methanohalophilus mahii
ATP
-
 Methanohalobium evestigatum
ATP
-
 Methanosarcina spp.

General Information

General Information Comment Organism
evolution PylC has similarity to members of the carbamoyl phosphate synthetase family, including D-Ala, D-Ala ligase, and amide formation is in keeping with this phylogeny Methanococcoides burtonii
evolution PylC has similarity to members of the carbamoyl phosphate synthetase family, including D-Ala, D-Ala ligase, and amide formation is in keeping with this phylogeny Methanohalophilus mahii
evolution PylC has similarity to members of the carbamoyl phosphate synthetase family, including D-Ala, D-Ala ligase, and amide formation is in keeping with this phylogeny Methanohalobium evestigatum
evolution PylC has similarity to members of the carbamoyl phosphate synthetase family, including D-Ala, D-Ala ligase, and amide formation is in keeping with this phylogeny Methanosarcina spp.
metabolism in the Methanosarcinaceae, and related species such as Methanococcoides burtonii, Methanohalophilus mahii, and Methanohalobium evestigatum, pyrrolysine is synthesized and incorporated into the methylamine methyltransferases, involved in metabolism of trimethylamine, dimethylamine, or monomethylamine, through the combined actions of the products of the pyl genes. The pylT gene encodes tRNAPyl whose CUA anticodon allows for amber codon translation. The pylS gene produces the pyrrolysyl-tRNA synthetase that charges tRNAPyl directly with pyrrolysine. The synthesis of pyrrolysine is carried out by the pylBCD gene products. Metabolism overview Methanococcoides burtonii
metabolism in the Methanosarcinaceae, and related species such as Methanococcoides burtonii, Methanohalophilus mahii, and Methanohalobium evestigatum, pyrrolysine is synthesized and incorporated into the methylamine methyltransferases, involved in metabolism of trimethylamine, dimethylamine, or monomethylamine, through the combined actions of the products of the pyl genes. The pylT gene encodes tRNAPyl whose CUA anticodon allows for amber codon translation. The pylS gene produces the pyrrolysyl-tRNA synthetase that charges tRNAPyl directly with pyrrolysine. The synthesis of pyrrolysine is carried out by the pylBCD gene products. Metabolism overview Methanohalophilus mahii
metabolism in the Methanosarcinaceae, and related species such as Methanococcoides burtonii, Methanohalophilus mahii, and Methanohalobium evestigatum, pyrrolysine is synthesized and incorporated into the methylamine methyltransferases, involved in metabolism of trimethylamine, dimethylamine, or monomethylamine, through the combined actions of the products of the pyl genes. The pylT gene encodes tRNAPyl whose CUA anticodon allows for amber codon translation. The pylS gene produces the pyrrolysyl-tRNA synthetase that charges tRNAPyl directly with pyrrolysine. The synthesis of pyrrolysine is carried out by the pylBCD gene products. Metabolism overview Methanohalobium evestigatum
metabolism in the Methanosarcinaceae, and related species such as Methanococcoides burtonii, Methanohalophilus mahii, and Methanohalobium evestigatum, pyrrolysine is synthesized and incorporated into the methylamine methyltransferases, involved in metabolism of trimethylamine, dimethylamine, or monomethylamine, through the combined actions of the products of the pyl genes. The pylT gene encodes tRNAPyl whose CUA anticodon allows for amber codon translation. The pylS gene produces the pyrrolysyl-tRNA synthetase that charges tRNAPyl directly with pyrrolysine. The synthesis of pyrrolysine is carried out by the pylBCD gene products. Metabolism overview Methanosarcina spp.
physiological function synthesis of pyrrolysine is carried out by the pylBCD gene products. Formation of desmethylpyrrolysine (dmPyl) requires only PylC and PylD. PylC carries out ligation of D-ornithine to the terminal amine of lysine, as cells transformed with pylC produce D-ornithyl-epsilonN-lysine dependent on exogenous D-ornithine Methanococcoides burtonii
physiological function synthesis of pyrrolysine is carried out by the pylBCD gene products. Formation of desmethylpyrrolysine (dmPyl) requires only PylC and PylD. PylC carries out ligation of D-ornithine to the terminal amine of lysine, as cells transformed with pylC produce D-ornithyl-epsilonN-lysine dependent on exogenous D-ornithine Methanohalophilus mahii
physiological function synthesis of pyrrolysine is carried out by the pylBCD gene products. Formation of desmethylpyrrolysine (dmPyl) requires only PylC and PylD. PylC carries out ligation of D-ornithine to the terminal amine of lysine, as cells transformed with pylC produce D-ornithyl-epsilonN-lysine dependent on exogenous D-ornithine Methanohalobium evestigatum
physiological function synthesis of pyrrolysine is carried out by the pylBCD gene products. Formation of desmethylpyrrolysine (dmPyl) requires only PylC and PylD. PylC carries out ligation of D-ornithine to the terminal amine of lysine, as cells transformed with pylC produce D-ornithyl-epsilonN-lysine dependent on exogenous D-ornithine Methanosarcina spp.